姜黄素致SW872脂肪细胞凋亡作用
Effect of curcumin on apoptosis and its mechanisms in SW872 adipocytes
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摘要: 目的 探讨姜黄素对SW872脂肪细胞凋亡的影响及其凋亡机制。方法 MTT法检测不同剂量姜黄素对SW872前脂肪细胞和成熟脂肪细胞生长抑制的影响;4',6-二脒基-2-苯基吲哚(DAPI)染色观察姜黄素作用后成熟脂肪细胞内细胞核变化。蛋白印迹法检测聚腺苷二磷酸核糖聚合酶(PARP)裂解产物和Bcl-2-associated X protein(Bax)表达量。结果 姜黄素对SW872前脂肪细胞及成熟脂肪细胞生长有抑制作用,并呈剂量-时间效应关系,不同剂量姜黄素作用成熟脂肪细胞72 h,与对照组比较,抑制率分别为2.97%、3.48%、19.76%、85.03%和94.48%;DAPI染色镜下观察成熟脂肪细胞出现明显核皱缩、变形现象;随姜黄素剂量增加,PARP裂解产物Bax蛋白表达量呈上升趋势(P<0.01)。结论 姜黄素促进SW872脂肪细胞凋亡,凋亡机制与线粒体密切相关。Abstract: Objective To explore the effect of curcumin on the apoptosis of SW872 adipocytes and the molecular mechanisms of the apoptosis. Methods The proliferation potential of SW872 preadipocytes and mature adipocytes treated with different concentrations of curcumin were evaluated by methyl thiazol tetrazolium(MTT)methods.The apoptosis of SW872 mature adipocytes were determined with 4',6-diamidino-2-phenylindole(DAPI)staining.Western blot was used to detect the expression of poly(ADP-ribolse)polymerase(PARP)cleavage and Bax. Results Curcumin could significantly inhibit the replication of SW872 preadipocytes in a dose-and time-dependent manner.Curcumin could inhibit the viability of SW872 mature adipocytes in a dose-and time-dependent manner.We observed nuclear shrinkage and deformation in SW872 mature adipocytes by DAPI staining.Curcumin could cause the increases in PARP cleavage and Bax expression in a dose-dependent manner. Conclusion Curcumin mediates adipocyte apoptosis via a mitochondrial pathw ay in SW872 adipocytes.
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