Abstract: Objective To evaluate the reliability and validity of a self-designed Questionnaire on Health Status Assessment. Methods Data were collected from 3 187 participants aged 18-55 years in Hubei province with the questionnaire. The construct validity was evaluated by exploratory factor analysis and Cronbach alpha coefficients were estimated to investigate the homogeneity reliability. Results Exploratory factor analysis revealed that the questionnaire contained soma-psychological-social scale(SPSS),fatigue assessment scale(FAS)and sleep quality scale(SQS).The SPSS was consisted of a social support factor,a vigour factor,and a pain factor.These factors accounted for 55.37% of the variance on the SPSS. FAS and SQS was consisted of a fatigue factor and a sleep quality factor,respectively,which accounted for 67.94% and 41.95% of the variance on the FAS and SQS.The correlation coefficient of these 3 sub-scale with the MOS 36-item shortform health survey(SF-36)was 0.633,0.363,and 0.457,respectively.The Cronbach alpha coefficients of sub-scales varied between 0.643-0.875. Conclusion The reliability and validity of the Questionnaire on Health Status Assessment are good and the questionnaire could be used as an effective tool to measure the status of sub-health among Chinese adults.