围产期暴露任基酚对子代大鼠脂肪组织形成影响

张宏宇; 刘志国; 柯晓煜

doi:10.11847/zgggws1113793

围产期暴露任基酚对子代大鼠脂肪组织形成影响

Effect of perinatal exposure to 4-nonylphenol on adipogenesis in offspring rats

摘要

摘要:
目的探讨围产期暴露壬基酚对子代大鼠脂肪组织的形成以及对大鼠脂肪组织中ERα表达的影响。
方法雌性大鼠从妊娠期（GD）第6 d至分娩后21 d分别给予壬基酚（5、25、125 μg/kg），另设一对照组，每天灌胃1次。子代出生后（PND）21 d时，处死部分幼鼠，收集血清；剩余部分幼鼠按性别分笼喂养，给予普通饲料至实验结束。23周后，断头处死，收集血液，并迅速取出肾周和性腺周围脂肪组织。
结果与对照组雄性（384 ± 43）g、雌性（231 ± 8）g比较，5、25、125 μg/kg壬基酚组子代大鼠体重雄性分别为（453 ± 51）、（475 ± 57）、（509 ± 29）g，雌性分别为（260 ± 13）、（260 ± 12）、（284 ± 3）g均升高（均P < 0.05）；与对照组比较，各计量壬基酚组子代大鼠血清中瘦素的水平均升高（均P < 0.05）；与对照组雄性（6 307 ± 312）μm²、雌性（5 915 ± 287）μm²比较，5、25、125 μg/kg壬基酚组子代大鼠脂肪细胞面积雄性分别为（10 142 ± 9 209）、（17 465 ± 9 830）、（33 854 ± 2 620）μm²，雌性分别为（10 100 ± 9 095）、（15 134 ± 965）、（31 280 ± 4 654）μm²均增加（均P < 0.05）；与对照组比较，各剂量任基酚组子代大鼠脂肪分化和增殖基因表达均增加，子代大鼠脂肪组织中ERα 基因和蛋白表达水平均降低（均P < 0.05）。
结论围产期暴露壬基酚可以导致子代大鼠肥胖，脂肪组织中ERα基因和蛋白表达的降低可能是壬基酚的作用机制之一。

Abstract:
Objective To investigate the effect of 4-nonylphenol (4-NP) exposure of maternal rats during and after gestation period on adipogenesis and the expression of estrogen receptor alpha (ERα) in offspring rats.
Methods The female Wistar rats were exposed to 4-NP at the dosages of 5, 25, and 125 μg/kg by gavage once a day from the 6th gestation day to 21th postnatal day (PND). The serum samples of some of the offspring rats were collected at the 21th PND and the blood and adipose tissues adjacent to kidney and gonads of the other offspring rats were collected 23 weeks after their births. A control group with 16 rats treated the same as the exposed rats but without 4-NP was established simultaneously.
Results The body weight of the offspring rats with maternal exposure to 5, 25, and 125 μg/kg 4-NP were all significantly higher than those of the control rats (male offspring rats: 453 ± 51, 475 ± 57, and 509 ± 29 g vs. 384 ± 43 g, female offspring rats: 260 ± 13, 260 ± 12, and 284 ± 3 g vs. 231 ± 8 g) (P < 0.05 for all). The serum total cholesterol and leptin of the offspring rats with maternal 4-NP exposure were significantly higher than those of the control rats (P < 0.05 for all). Compared to those of the control rats, the areas of adipose tissues of the offspring rats with 5, 25, and 125 μg/kg maternal 4-NP exposure significantly increased (male offspring rats: 10 142 ± 9 209, 17 465 ± 9 830, and 33 854 ± 2 620 μm² vs. 6 307 ± 312 μm², female offspring rats: 10 100 ± 9 095, 15 134 ± 965, and 31 280 ± 4 654 μm² vs. 5 915 ± 287 μm²) (all P < 0.05). Compared with those of the control rats, the expressions of the genes related to the differentiation and proliferation of adipocytes significantly increased (P < 0.05 for all), while the expressions of ERα mRNA and protein were down-regulated for the offspring rats with 4-NP exposure of various dosages.
Conclusion Maternal exposure to 4-NP during and after gestation period can affect adipogenesis of both male and female offspring rats and down-regulated expressions of ERα gene and protein may related to the effects.

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