汉滩型汉坦病毒环介导等温扩增（LAMP）检测方法建立

Establishment of a loop mediated isothermal amplification assay for detection of Hantaan virus

摘要:
目的建立一种针对汉滩型汉坦病毒的环介导等温扩增（LAMP）检测技术，以实现汉坦病毒的快速、高效检测。
方法针对汉坦病毒汉滩型S片段的保守区，设计合成4条特异性引物，建立汉滩型汉坦病毒LAMP检测方法。
结果该方法能够有效检测汉坦病毒标准质粒，并且该套引物具有良好的特异性，检测灵敏度达到10个基因拷贝，应用建立的LAMP检测方法能够有效检测血液样本中的汉滩型汉坦病毒。
结论建立的LAMP方法具有高度灵敏性及特异性，是一种高效快速的检测汉滩型汉坦病毒的基因检测方法。

Abstract:
Objective To establish a new method for rapid and efficient detection of Hantaan virus with loop mediated isothermal amplification (LAMP) technique.
Methods Four specific primers were designed and synthesized according to conserved region of Hantaan virus S fragment and a LAMP detection method was established.
Results The established method can effectively detect Hantaan virus standard plasmid and the primers have good specificity for the detection of Hantaan virus. The detection sensitivity of LAMP is 10 copies per reaction. And the established LAMP method can detect Hantaan virus in blood samples effectively.
Conclusion The established LAMP method is highly sensitive and specific, and it is an efficient and rapid method for the detection of Hantaan virus.