Abstract:
Objective To analyze intestinal distribution of Eubacterium rectale and Lactobacillus in normal-weight, overweight and obese school-age children and the characteristics of intestinal flora imbalance in overweight and obese children and to provide a theoretical basis for the prevention and control of obesity through intestinal microecological regulation.
Methods We collected 166 feces samples from all pupils in a primary school in Nanjing city of Jiangsu province for detections of Eubacterium rectale and Lactobacillus with real-time quantitative PCR method.
Results Significant differences in the number of Eubacterium rectale and Lactobacillus were observed among the three groups of children, with a higher number in the obese children and a lower number in the normal-weight children for Eubacterium rectale (P < 0.05) but a higher number in the normal-weight children and a lower number in the obese children for Lactobacillus (P < 0.05), respectively. No significant gender difference in the number of intestinal Eubacterium rectale and Lactobacillus was observed among all the three groups of children. The number of Lactobacillus decreased significantly with the increment of age only in the normal-weight children (P < 0.05) but no age difference was observed in the number of intestinal Eubacterium rectale among the three groups and in Lactobacillus number in overweight and obese children.
Conclusion The increase in the number of Eubacterium rectale and the decrease in the number of intestinal Lactobacillus may be related to the occurrence and development of overweight and obesity in school age children. The distribution of Eubacterium rectale and Lactobacillus in the intestine of normal weight, overweight and obese children are less affected by children′s gender and age.
下载: