Objective  To analyze the effect of non-governmental organization (NGO) involvement in prevention and treatment of human immunodeficiency virus infection/acquired immunodeficiency syndrome (HIV/AIDS) among men who have sex with men (MSM) under the “three-pronged approach” strategy (management on HIV/AIDS cases identification and treatment comprehensively implemented by disease prevention and control centers, medical institutions, and NGO) and to provide evidences for developing HIV/AIDS prevention and treatment strategies.

  Methods  The data of study were derived from United States Centers for Disease Control and Prevention Global AIDS Program – China Cooperation (GAP) Program – the intervention was implemented with involvements of NGO between April 2017 and March 2019 in three municipalities of Guangxi Zhuang Autonomous Region (Guangxi) and the aim of the intervention was to meet the Joint United Nations Programme on HIV and AIDS (UNAIDS) 90 – 90 – 90 target (to diagnose 90% of all HIV positive people, provide antiretroviral therapy ART for 90% of those diagnosed and achieve viral suppression for 90% of those treated, by 2020). The collected data included monthly number of HIV screening test, HIV positive cases, HIV confirmation test (Western blot, WB), HIV positive cases confirmed by WB, HIV/AIDS cases enrolled for ART, viral load detections among cases with ART, and cases with viral suppression after ART.

  Results  During the three-year period in the three municipalities, totally 8 712 MSM received HIV screening tests and the HIV detection rate was 46.99% among the 18 541 MSM covered by the intervention program. Among the examinees of HIV screening test, 570 were HIV positive, of them, 457 (80.18%) received WB tests and 443 had positive confirmation result. For the 443 WB-confirmed HIV positive, 383 (86.46%) were success-fully referred for ART and viral suppression was achieved among nearly all (97.13%) of the cases receiving ART.

  Conclusion  The involvement of NGO in comprehensive HIV intervention program among MSM can effectively promote the diagnosis of HIV infections and management of cases living with HIV/AIDS to achieve the 90 – 90 – 90 targets.