Objective  To explore human immunodeficiency virus (HIV) antibody testing behavior and its influencing factors among university students with sexual experience and provide evidences for developing relevant education programs and promoting HIV testing in the students.

  Methods  Based on the United Sates Centers for Disease Control and Prevention Global AIDS Program – China Cooperation (GAP) conducted in 2015, we carried out an online questionnaire survey among undergraduates and graduates recruited with convenient and snowball sampling in 5 universities in Beijing city. From the participants of the survey, 355 students aged 16 years and above and reporting sexual behaviors were included in the analysis.

  Results  Of the 355 students, 10.14% had HIV antibody testing. Multivariate logistic regression analysis demonstrated that the students ever having anal sex and meeting their sexual partners at public entertainment places were more likely to have HIV antibody testing compared to those never having anal sex and meeting their sexual partners at places other than entertainment places, with the adjusted odds ratio of 4.022 and 9.298. Stratified logistic regression analysis revealed that for the students with an urban birthplace and with more than two sexual partners, the male students and those ever having anal sex were more likely to have HIV antibody testing.

  Conclusion  The HIV-related practice is not consistent with relevant knowledge and attitude and among university students with sexual experience and the male students with anal sex history are more likely to have HIV antibody testing than other students. The results suggest the importance of early education on HIV prevention among adolescents in various regions.