疟原虫棒状复合体红细胞结合受体基因筛选与生物信息学分析

张铭宇; 丁祎; 郑丽

doi:10.11847/zgggws1126954

疟原虫棒状复合体红细胞结合受体基因筛选与生物信息学分析

Screening and bioinformatics analysis on genes associated with erythrocyte receptor of Plasmodium RhopH complex

摘要

摘要:
目的利用生物信息学预测RhopH complex相应红细胞受体的候选基因，为防治疟疾提供依据。
方法对小鼠11号染色体D11Mit10和D11Mit284区间表达的基因进行红细胞转录、信号肽、跨膜区以及GPI结构评估。
结果小鼠11号染色体D11Mit10 和D11Mit284区间表达的基因共339个，通过红细胞蛋白组学数据库筛选出137个在红细胞表达的基因，进一步分析137个红细胞蛋白组基因发现拥有跨膜区的7个，具有信号肽的4个，但均不具有GPI位点。Atp5g1、Slc4a1、Itgb3等基因可能表达在红细胞膜表面，具有成为棒状体蛋白结合受体的可能。
结论利用NCBI、Uniprot、ExPASy-PortParam等网络资源评估以Slc4a1和Itgb3为代表的基因可能为RhopH complex与红细胞结合的受体基因。

Abstract:
Objective To predict candidate genes associated with red blood cell receptor of Plasmodium RhopH complex using bioinformatics analysis for providing evidences to malaria prevention.
Methods Using National Center for Biotechnology Information (NCBI), Uniprot, ExPASy-PortParam and other network resources, the mouse genes in D11Mit10 and D11Mit284 regions on chromosome 11 were assessed for erythrocyte transcription, signal peptide, trans-membrane region and glycosylpbosphatidylinositol (GPI) structure.
Results A total of 339 expressed genes on the mouse chromosome 11 were identified, of which 137 were transcribed and expressed in red blood cells; 7 had trans-membrane regions, 4 had signal peptides, but none had GPI sites. Some of the genes as Atp5g1, Slc4a1, Itgb3 may be expressed on the surface of red blood cell membrane and their expressions may be correlated with erythrocyte receptor for Plasmodium RhopH complex.
Conclusion The expression of some genes such as Slc4a1 and Itgb3 on mouse chromosome 11 may be correlated with the erythrocyte receptor of Plasmodium RhopH complex.

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