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ShRNA干扰G蛋白偶联受体相关分选蛋白1对肺癌细胞增殖凋亡和裸鼠成瘤影响

韩贵良 石岩 焦婷 贾友超 任冠颖

韩贵良, 石岩, 焦婷, 贾友超, 任冠颖. ShRNA干扰G蛋白偶联受体相关分选蛋白1对肺癌细胞增殖凋亡和裸鼠成瘤影响[J]. 中国公共卫生, 2021, 37(6): 978-981. doi: 10.11847/zgggws1129849
引用本文: 韩贵良, 石岩, 焦婷, 贾友超, 任冠颖. ShRNA干扰G蛋白偶联受体相关分选蛋白1对肺癌细胞增殖凋亡和裸鼠成瘤影响[J]. 中国公共卫生, 2021, 37(6): 978-981. doi: 10.11847/zgggws1129849
HAN Gui-liang, SHI Yan, JIAO Ting, . Effect of GASP-1-ShRNA on proliferation and apoptosis of lung cancer cells and tumor formation in nude mice[J]. Chinese Journal of Public Health, 2021, 37(6): 978-981. doi: 10.11847/zgggws1129849
Citation: HAN Gui-liang, SHI Yan, JIAO Ting, . Effect of GASP-1-ShRNA on proliferation and apoptosis of lung cancer cells and tumor formation in nude mice[J]. Chinese Journal of Public Health, 2021, 37(6): 978-981. doi: 10.11847/zgggws1129849

ShRNA干扰G蛋白偶联受体相关分选蛋白1对肺癌细胞增殖凋亡和裸鼠成瘤影响

doi: 10.11847/zgggws1129849
基金项目: 2019年保定市科技计划自筹经费(1941ZF084)
详细信息
    作者简介:

    韩贵良(1981 – ),男,主治医师,硕士,研究方向:肺癌早期诊断和规范化治疗

    通信作者:

    任冠颖,E-mail:renguanying1982@126.com

  • 中图分类号: R 99

Effect of GASP-1-ShRNA on proliferation and apoptosis of lung cancer cells and tumor formation in nude mice

  • 摘要:   目的  探讨shRNA干扰G蛋白偶联受体相关分选蛋白1(GASP-1)对肺癌细胞增殖凋亡和裸鼠成瘤影响。  方法  将GASP-1-shRNA1、2和3用Lipofectamine 2 000转染到肺癌A549细胞。选择转染GASP-1-shRNA1的细胞,建立空白对照组、空载体转染组和GASP-1-shRNA1转染组,采用菌落形成试验和流式细胞术分别检测肺癌A549细胞增殖和调亡;采用Western Blot检测ki67、cleaved caspase-3、Bax和Bcl-2蛋白表达量。将转染GASP-1-shRNA1细胞注射裸鼠后腿建立原位异种移植小鼠模型。检测肿瘤的生长,并通过免疫组化检测ki67和caspase-3蛋白表达量。  结果  GASP-1-shRNA1、2和3转染A549细胞后GASP-1 mRNA和蛋白表达量均显著下调,尤其转染GASP-1-shRNA1组。GASP-1下调能抑制A549细胞增殖,并促进A549细胞凋亡;在体内试验中,下调GASP-1能抑制体内肿瘤生长,同时也能抑制细胞增殖,并促进细胞凋亡。  结论  GASP-1的下调可抑制肺癌细胞增殖、促进凋亡,并抑制肿瘤生长。
  • 图  1  ShRNA转染对GASP-1蛋白表达影响

    注:1对照组;2 shRNA-NC;3~5 GASP-1-shRNA 1、2、3组。

    图  2  细胞增殖试验(菌落形成试验)

    图  3  GASP-1-shRNA1对凋亡相关蛋白表达影响

    注:1 对照组;2 shRNA-NC组;3 GASP-1-shRNA1组。

    图  4  GASP-1-shRNA1对肺癌模型小鼠影响

    :A肿瘤体积变化;B ki67和caspase-3蛋白表达量(免疫组化)。

    表  1  ShRNA转染对GASP-1 mRNA和蛋白表达影响($\overline x $ ± sn = 8)

    组别GASP-1 mRNA表达量GASP-1蛋白表达量
    对照组 1.00 ± 0.00 0.131 ± 0.024
    shRNA-NC 0.99 ± 0.05 0.125 ± 0.033
    GASP-1-shRNA 1 0.15 ± 0.07 a 0.006 ± 0.005 a
           2 0.64 ± 0.09 b 0.048 ± 0.014 b
           3 0.41 ± 0.06 b 0.260 ± 0.015 b
      注:与shRNA-NC组比较,a P < 0.01,b P < 0.05。
    下载: 导出CSV
  • [1] Reck M, Popat S, Reinmuth N, et al. Metastatic non-small-cell lung cancer (NSCLC): ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up[J]. Annals of Oncology, 2014, 25(Suppl 3): iii27 – iii39.
    [2] Wen CH, Dehnel T. China wrestles with lung cancer[J]. The Lancet Oncology, 2011, 12(1): 15. doi: 10.1016/S1470-2045(10)70303-X
    [3] Lv SG, Xue J, Wu CY, et al. Identification of a panel of serum microRNAs as biomarkers for early detection of lung adenocarcinoma[J]. Journal of Cancer, 2017, 8(1): 48 – 56. doi: 10.7150/jca.16644
    [4] Zhou YY, Shen SM. MiR-520f acts as a biomarker for the diagnosis of lung cancer[J]. Medicine, 2019, 98(30): e16546. doi: 10.1097/MD.0000000000016546
    [5] Świtlik W, Karbownik MS, Suwalski M, et al. miR-30a-5p together with miR-210 -3p as a promising biomarker for non-small cell lung cancer: a preliminary study[J]. Cancer Biomarkers, 2018, 21(2): 479 – 488. doi: 10.3233/CBM-170767
    [6] Hill JJ, Qiu YC, Hewick RM, et al. Regulation of myostatin in vivo by growth and differentiation factor-associated serum protein-1: a novel protein with protease inhibitor and follistatin domains[J]. Molecular Endocrinology, 2003, 17(6): 1144 – 1154. doi: 10.1210/me.2002-0366
    [7] Walker RG, Angerman EB, Kattamuri C, et al. Alternative binding modes identified for growth and differentiation factor-associated serum protein (GASP) family antagonism of myostatin[J]. The Journal of Biological Chemistry, 2015, 290(12): 7506 – 7516. doi: 10.1074/jbc.M114.624130
    [8] Zheng XY, Chang F, Zhang XM, et al. G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker[J]. Experimental and Molecular Pathology, 2012, 93(1): 111 – 115. doi: 10.1016/j.yexmp.2012.03.013
    [9] Li Y, Yu HY, Han FF, et al. Biochanin A induces S phase arrest and apoptosis in lung cancer cells[J]. Biomed Research International, 2018, 2018: 3545376.
    [10] Bar-Shavit R, Maoz M, Kancharla A, et al. G protein-coupled receptors in cancer[J]. International Journal of Molecular Sciences, 2016, 17(8): 1320. doi: 10.3390/ijms17081320
    [11] Lappano R, Jacquot Y, Maggiolini M. GPCR modulation in breast cancer[J]. International Journal of Molecular Sciences, 2018, 19(12): 3840. doi: 10.3390/ijms19123840
    [12] Dong DD, Zhou H, Li G. GPR78 promotes lung cancer cell migration and metastasis by activation of Gαq-Rho GTPase pathway[J]. BMB Reports, 2016, 49(11): 623 – 628. doi: 10.5483/BMBRep.2016.49.11.133
    [13] Folescu R, Levai CM, Grigoraş ML, et al. Expression and significance of Ki-67 in lung cancer[J]. Romanian Journal of Morphology and Embryology, 2018, 59(1): 227 – 233.
    [14] Hengartner MO. The biochemistry of apoptosis[J]. Nature, 2000, 407(6805): 770 – 776. doi: 10.1038/35037710
    [15] Thornberry NA, Lazebnik Y. Caspases: enemies within[J]. Science, 1998, 281(5381): 1312 – 1316. doi: 10.1126/science.281.5381.1312
    [16] Liu GB, Pei F, Yang FQ, et al. Role of autophagy and apoptosis in non-small-cell lung cancer[J]. International Journal of Molecular Sciences, 2017, 18(2): 367. doi: 10.3390/ijms18020367
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出版历程
  • 接收日期:  2020-04-05
  • 网络出版日期:  2021-06-29
  • 刊出日期:  2021-06-03

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