Gene frequency of MNS and Mur blood group system in Li ethnic group in Hainan province: distribution and comparison with other ethnic groups
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摘要:
目的 了解海南黎族人群MNS和Mur血型系统基因频率分布及其与其他民族人群的差异,为稀有血型库的建立及临床输血提供参考依据。 方法 于2020年1月在海南省琼中县黎母山地区采集300份(男性180份、女性120份)三代以内均为黎族的18~60岁志愿者静脉血3~5 mL,采用聚合酶链式反应 – 序列特异性引物法(PCR-SSP)对MNS、Mur血型系统等位基因进行检测和分型,分析其基因频率并与其他民族人群进行比较。 结果 海南300名黎族人群的MNS血型系统中,MM表型199人,MN表型93人,NN表型8人,SS表型1人,Ss表型15人,ss表型284人,等位基因频率为M = 0.8183、N = 0.1817、S = 0.0283、s = 0.9717;与湖南岳阳汉族人群、四川成都汉族人群、山西运城汉族人群、新疆回族人群、新疆维吾尔族人群、新疆哈萨克族人群、西藏藏族人群和四川彝族人群的MNS血型等位基因频率比较,差异均有统计学意义(均P < 0.01)。海南300名黎族人群的Mur血型系统中,Mur + 表型173人,Mur-表型127人,等位基因频率为Mur + = 0.5767、Mur – = 0.4233;与上海汉族人群、陕西西安汉族人群、广东番禺汉族人群、四川汉族人群、黑龙江哈尔滨满族人群和湖南岳阳汉族人群的Mur血型基因频率比较,差异均有统计学意义(均P < 0.001)。M/N和S/s对偶抗原的不配合率(MP)分别为25.76 %和5.35 %。 结论 海南黎族人群MNS和Mur血型系统基因遗传状况保持相对稳定,MNS血型基因频率呈多态性分布、Mur血型阳性基因频率较高,与国内其他不同民族相比具有独特的民族分布特征。 Abstract:Objective To investigate gene frequency of MNS and Mur blood group system in Li ethnic group in Hainan province and the differences in the genes′ distribution compared to other ethnic groups for providing references to the establishment of rare blood group bank and clinical blood transfusion. Methods The study was conducted in Limu Mountain area of Qiongzhong county in Hainan province during January 2020 and the voluntary participants were 300 permanent residents (180 males and 120 females aged 18 – 60 years) being Li nationality within three generations and without serious diseases and familial genetic diseases. Venous blood samples (3 – 5 ml for each sample) were collected from all the participants. MNS and Mur blood group alleles were detected and genotyped with sequence-specific primer polymerase chain reaction (SSP-PCR) and the gene frequencies were analyzed and compared with other ethnic groups in China. Results The number of participants with various phenotypes of MNS blood group were 199 for MM, 93 for MN, 8 for NN, 1 for SS, 15 for Ss, and 284 for ss; the allele frequencies were 0.8183 for M, 0.1817 for N, 0.0283 for S, and 0.9717 for s, respectively. The participants′ distributions of MNS blood group allele frequencies were significantly different from those of Han ethnic groups in Yueyang city of Hunan province/Chengdu city of Sichuan province/Yuncheng city of Shanxi province, Hui/Uygur/Kazak ethnic groups in Xinjiang Uygur Autonomous Region, Tibetan ethnic group in Tibet Autonomous Region, and Yi ethnic group in Sichuan province (P < 0.01 for all). The number of participants with various phenotypes of Mur blood group were 173 for Mur + , 127 for Mur –, with the allele frequencies of 0.5767 for Mur + and 0.4233 for Mur –; the participants′ Mur blood group allele frequencies were significantly different from those of Han ethnic groups in Shanghai city/Xi′an city of Shanxi province/Panyu city of Guangdong province/Sichuan province/Yueyang city of Hunan province and Man ethnic group in Harbin city of Heilongjiang province (P < 0.001 for all). The antigen incompatibility rates of M/N and S/s were 25.76% and 5.35%, respectively. Conclusion The genetic status of MNS and Mur blood group system genes remains relatively stable in Li ethnic group in Hainan province. The gene frequencies of MNS blood group system are polymorphic, and the frequency of Mur blood group positive gene is higher, indicating unique ethnic distribution characteristics compared with that in other ethnic groups in China. -
Key words:
- MNS /
- Mur /
- blood group system /
- gene frequency /
- Li ethnic group in Hainan province
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表 1 海南黎族人群MNS和Mur稀有血型系统基因频率分布的Hardy-Weinberg平衡检验(n = 300)
血型系统 表型 观察值 观察值频率 期望值 期望值频率 χ2 值 P 值 MNS M + N – 199 0.6633 200.88 0.6615 0.545 0.762 M – N + 8 0.0267 9.90 0.0348 M + N + 93 0.3100 89.21 0.3037 S + s – 1 0.0033 0.24 0.0008 2.541 0.816 S – s + 284 0.9467 283.26 0.9442 S + s + 15 0.0500 16.50 0.0550 Mur Mur + 173 0.5767 173.00 0.5767 0.000 1.000 Mur – 127 0.4252 127.00 0.4252 表 2 海南黎族人群与其他民族人群MNS血型系统等位基因频率比较
人群 标本数 等位基因频率 M频率 N频率 χ2 值 a P 值 a S频率 s频率 χ2 值 a P 值 a 海南黎族 300 0.8183 0.1817 0.0283 0.9717 湖南岳阳汉族[13] 303 0.4953 0.5107 70.431 < 0.001 0.0479 0.9521 2.143 0.143 四川成都汉族[14] 332 0.5752 0.4246 42.913 < 0.001 0.0376 0.9624 0.462 0.497 山西运城汉族[15] 300 0.5467 0.4533 50.392 < 0.001 0.0817 0.9813 8.189 0.004 新疆回族[16] 220 0.5204 0.4796 52.902 < 0.001 0.0954 0.9046 11.405 0.001 新疆维吾尔族[17] 158 0.5971 0.4209 29.182 < 0.001 0.1743 0.8009 33.256 < 0.001 新疆哈萨克族[18] 196 0.6454 0.3546 18.999 < 0.001 0.1431 0.8469 24.141 < 0.001 西藏藏族[19] 409 0.6809 0.3191 16.775 < 0.001 0.1467 0.8533 28.756 < 0.001 四川彝族[20] 120 0.6833 0.3167 8.839 0.003 0.0917 0.9083 8.385 0.004 注:a 与海南黎族人群比较,P < 0.05。 表 3 海南黎族人群与其他民族人群Mur血型基因频率比较
人群 标本数 Mur + 基因频率 Mur – 基因频率 χ2 值 a P 值 a 海南黎族 300 0.5767 0.4233 上海汉族[2] 1673 0.0179 0.9821 860.372 < 0.001 陕西西安汉族[3] 379 0.0105 0.9895 278.457 < 0.001 广东番禺汉族[4] 3000 0.0717 0.9283 670.372 < 0.001 四川汉族[5] 700 0.1142 0.8858 237.563 < 0.001 黑龙江哈尔滨满族[6] 200 0.0050 0.9950 172.838 < 0.001 广东惠州汉族[7] 1119 0.5610 0.4390 0.230 0.632 湖南岳阳汉族[13] 303 0.0363 0.9637 207.597 < 0.001 注:a 与海南黎族人群比较,P < 0.05。 -
[1] Storry JR, Clausen FB, Castilho L, et al. International Society of Blood Transfusion Working Party on red cell immunogenetics and blood group terminology: report of the Dubai, Copenhagen and Toronto meetings[J]. Vox Sanguinis, 2019, 114(1): 95 – 102. doi: 10.1111/vox.12717 [2] 龚淞颂, 沈伟, 王钰箐, 等. 中国部分人群Mur血型抗原分布及分子基础的研究[J]. 中国输血杂志, 2015, 28(8): 997 – 1000. [3] 王红, 吴大洲, 张薇薇, 等. Mur基因分型方法的建立及西安地区无偿献血者的频率分布[J]. 中国输血杂志, 2018, 31(4): 374 – 376. [4] 谢敬文, 蓝文莉, 严康峰, 等. 献血人群Mur血型的筛查研究[J]. 临床输血与检验, 2016, 18(4): 384 – 386. doi: 10.3969/j.issn.1671-2587.2016.04.027 [5] 李翠莹, 徐弘, 黄菲, 等. 四川汉族人群Mur抗原频率调查[J]. 临床血液学杂志, 2015, 28(12): 1078 – 1079. [6] 刘颖, 毕冬梅, 赵素珍, 等. 中国哈尔滨地区满族人群11个红细胞血型系统24种稀有血型抗原的基因多态性研究[J]. 中国实验血液学杂志, 2017, 25(6): 1799 – 1803. [7] 严凤好, 朱春燕, 李雪群, 等. 惠州地区无偿献血者抗 – Mur筛查及Mur抗原频率分布[J]. 广州医药, 2015, 46(3): 75 – 77. doi: 10.3969/j.issn.1000-8535.2015.03.024 [8] 王荣鹏, 郑洋洋, 陈凤. 内蒙古地区蒙古族人群12种稀有血型系统基因频率调查研究[J]. 内蒙古医学杂志, 2021, 53(6): 641 – 645,651. [9] 邹新, 虞茜, 葛平英, 等. 常州地区Rh、MNS血型不合致新生儿溶血病的试验结果分析及治疗[J]. 检验医学与临床, 2021, 18(9): 1185 – 1188. doi: 10.3969/j.issn.1672-9455.2021.09.001 [10] 陆奉科, 莫柱宁, 黎海澜, 等. 某地区抗Mur抗体分布频率的调查研究及输血策略[J]. 国际检验医学杂志, 2016, 37(20): 2945 – 2946. doi: 10.3969/j.issn.1673-4130.2016.20.067 [11] 贺锋, 程金凤, 蔡细英. MNS意外抗体检测及对血型鉴定和交叉配血影响的研究[J]. 临床血液学杂志, 2018, 31(10): 746 – 749. [12] 焦立新, 陈琳, 杨帆, 等. 抗 – S引起新生儿溶血病1例[J]. 中国实验诊断学, 2016, 20(3): 509 – 510. [13] 孙昂, 苏湘晖, 陈宏伟, 等. 岳阳汉族人群10个红细胞血型系统基因频率及多态性研究[J]. 中国输血杂志, 2020, 33(10): 1033 – 1037. [14] 洪缨, 巩天祥, 周昌华, 等. 成都地区献血人群Kell等9个血型系统抗原基因分型研究[J]. 中国输血杂志, 2012, 25(8): 763 – 766. [15] 方斐, 周永安, 李超, 等. 运城地区汉族人群稀有血型基因多态性分析[J]. 中国优生与遗传杂志, 2018, 26(6): 36 – 38. [16] 林国跃, 单金晶, 张雅楠, 等. 中国新疆回族人群9种稀有血型系统基因频率调查研究[J]. 新疆医科大学学报, 2016, 39(8): 1026 – 1031. doi: 10.3969/j.issn.1009-5551.2016.08.022 [17] 林国跃, 杜小璐, 单金晶, 等. 中国新疆维吾尔族人群MNS、Duffy和Kell等稀有血型的基因分子遗传分析[J]. 中国组织工程研究, 2016, 20(1): 123 – 127. doi: 10.3969/j.issn.2095-4344.2016.01.022 [18] 虞彬, 单金晶, 张雅楠, 等. 中国新疆哈萨克族人9种稀有血型系统基因频率分布状况[J]. 解放军预防医学杂志, 2018, 36(1): 4 – 10. [19] 张嵘, 田力, 李晓娟, 等. 西藏藏族人群多个红细胞血型系统基因多态性研究[J]. 中国输血杂志, 2014, 27(5): 505 – 507. [20] 辛友盼, 田力, 宋宁, 等. 四川地区彝族ABO等8个血型的分子遗传学研究[J]. 中国输血杂志, 2013, 26(1): 41 – 45. [21] Owaidah AY, Naffaa NM, Alumran A, et al. Phenotype frequen-cies of major blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran) among blood donors in the Eastern region of Saudi Arabia[J]. Journal of Blood Medicine, 2020, 11: 59 – 65. doi: 10.2147/JBM.S236834 [22] Gholamrezazade A, Amirizadeh N, Oodi A. Genotyping analysis of the MNS blood group system of thalassemia patients with alloanti-bodies in Iran[J]. Transfusion and Apheresis Science, 2021, 60(1): 103006. doi: 10.1016/j.transci.2020.103006 [23] 周娟, 吕蓉, 朱帮强, 等. 安徽省汉族人群Miltenberger血型调查分析[J]. 安徽医科大学学报, 2014, 49(3): 313 – 315. [24] 魏玲, 姬艳丽, 莫春妍, 等. 广州地区无偿献血者抗 – Mur筛查及Mur抗原基因型检测[J]. 南方医科大学学报, 2012, 32(12): 1833 – 1835. doi: 10.3969/j.issn.1673-4254.2012.12.033 [25] 张璐, 袁红, 伍钢. 抗M抗体、抗Lea抗体以及自身冷抗体引起的输血交叉配血不合1例[J]. 临床血液学杂志, 2017, 30(4): 325 – 326. [26] 古明高, 吴昌松, 罗庆艳, 等. 多次不良妊娠史合并抗 – C、抗 – M多重抗体孕妇成功分娩[J]. 实用医学杂志, 2017, 33(20): 3500. doi: 10.3969/j.issn.1006-5725.2017.20.045 [27] 吴红, 唐绮, 刘强. 低频率抗 – Mur引起的疑难配血1例[J]. 中国输血杂志, 2014, 27(1): 80 – 82.
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