Molecular and epidemiological characteristics of adenovirus isolated in patients with acute diarrhea in Ningxia, 2015 – 2020
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摘要:
目的 了解宁夏回族自治区腹泻患者腺病毒感染状况,流行病学特点和基因进化特征,为腺病毒所致疾病的科学防控提供理论依据。 方法 收集2015 — 2020年宁夏哨点监测医院门诊腹泻病例粪便样本,使用Real-time PCR方法筛选腺病毒阳性样本,通过PCR扩增腺病毒六邻体区基因,测序后进行病毒型别鉴定和基因进化分析。 结果 2015 — 2020年共收集腹泻病例样本6695份,共检测到腺病毒阳性样本278份(4.2 %)。其中,2017年腹泻样本数最多(1241份),且腺病毒阳性率最高(7.7 %,96份);腺病毒阳性率在不同季节和不同年龄组的差异均具有统计学意义(P < 0.05)。278份腺病毒阳性样本中,测序获得80份腺病毒基因序列,经鉴定有3种基因型别,以F41型为主,其次为F40型和C1型。 结论 腺病毒F41型是造成宁夏回族自治区肠道腺病毒性腹泻的优势型别,全年均有病例,全年龄组人群均易感。 Abstract:Objective To examine infection characteristics and genetic evolution of adenovirus isolated in patients with diarrhea in Ningxia Hui Autonomous Region (Ningxia) for providing evidence to the prevention and control of human adenovirus infection. Methods Totally 6 695 fecal specimens were collected from diarrheal outpatients at 10 diarrhea sentinel hospitals in 5 cities of Ningxia from 2015 through 2020. Adenovirus positive samples were screened out with real-time PCR. The gene of adenovirus hexon was amplified with PCR and sequenced for virus type identification and phylogenetic analysis. Results Of all the specimens detected, 4.2% (278) were adenovirus positive and the positive rate was the highest (7.7%) for the specimens collected in 2017. There were significant differences in the adenovirus positive rate among the specimens collected in different seasons and isolated from the outpatients of various age groups. Gene sequences were obtained for the 80 adenovirus strains isolated from the 278 viral positive specimens. For the 80 viral strains, three genotypes were identified and the main type was F41, followed by type F40 and type C1. Conclusion Adenovirus-induced diarrhea occured across a year among the people of various ages in Ningxia during 2015 – 2020 and the dominant adenovirus strain causing the diarrheal diseases was type F41. -
Key words:
- viral diarrhea /
- adenovirus /
- epidemic characteristics /
- gene characteristics
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表 1 2015 — 2020年不同季节腺病毒性腹泻分布情况
季节 样本数 阳性数 % χ2 值 P 值 春季 1758 48 2.7 15.759 < 0.05 夏季 2561 128 5.0 秋季 1568 74 4.7 冬季 808 28 3.5 合计 6695 278 4.2 表 2 2015 — 2020年不同月份腺病毒性腹泻分布(阳性率,%)
月份 2015年 2016年 2017年 2018年 2019年 2020年 样本数 阳性数 % 样本数 阳性数 % 样本数 阳性数 % 样本数 阳性数 % 样本数 阳性数 % 样本数 阳性数 % 1 11 1 9.1 55 0 0.0 36 0 0.0 80 1 1.3 81 2 2.5 54 6 11.1 2 2 0 0.0 47 0 0.0 58 1 1.7 70 3 4.3 48 1 2.1 48 1 2.1 3 31 2 6.5 58 1 1.7 108 2 1.9 94 3 3.2 77 3 3.9 47 4 8.5 4 35 0 0.0 95 1 1.1 65 1 1.5 172 2 1.2 105 1 1.0 133 3 2.3 5 123 0 0.0 90 3 3.3 143 11 7.7 144 1 0.7 119 6 5.0 119 4 3.4 6 145 3 2.1 100 1 1.0 201 19 9.5 116 0 0.0 138 6 4.3 136 3 2.2 7 130 7 5.4 167 1 0.6 183 23 12.6 134 0 0.0 164 13 7.9 141 13 9.2 8 96 0 0.0 201 2 1.0 128 15 11.7 125 1 0.8 156 9 5.8 100 12 12.0 9 57 4 7.0 117 2 1.7 92 5 5.4 68 3 4.4 108 3 2.8 95 5 5.3 10 90 4 4.4 114 1 0.9 75 5 6.7 61 0 0.0 108 0 0.0 96 12 12.5 11 101 4 4.0 49 0 0.0 94 10 10.6 44 1 2.3 100 2 2.0 99 13 13.1 12 28 0 0.0 27 1 3.7 58 4 6.9 19 0 0.0 46 4 8.7 40 3 7.5 合计 849 25 2.9 1120 13 1.2 1241 96 7.7 1127 15 1.3 1250 50 4.0 1108 79 7.1 表 3 2015 — 2020年不同特征人群腺病毒性腹泻检出情况
特征 组别 样本数 阳性数 % χ2 值 P 值 性别 男性 3774 165 4.4 1.049 0.168 女性 2921 113 3.9 年龄组(岁) 0~ 1768 94 5.3 62.833 < 0.05 1~ 1463 84 5.7 2~ 404 31 7.7 3~ 218 11 5.0 4~ 162 4 2.5 5~ 437 4 0.9 10~ 451 11 2.4 20~ 347 8 2.3 30~ 301 7 2.3 40~ 288 7 2.4 50~ 275 4 1.5 60~ 282 8 2.8 70~ 299 5 1.7 -
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