Abstract:
To investigate the distribution of HCV infection and subtypes among blood donors in some areas of Shandong Province.
MethodSerum samples were collected from 424 paid blood donors. Anti-HCV was detected by enzyme-linked immunosorbent assay (ELISA).Serotypes of anti-HCV positive sera were identified by ELISA and genotypes were determined by RT-PCR.
ResultsThe HCV prevalence of 424 blood donors was 21.70% (92/424). It was significantly higher in the donors donating blood and plasma (45.14%) than in the donors with only blood donation (6.94%). Of 92 anti-HCV positive sera detected, 32 (34.78%) were typed by ELISA. 30 (93.75%) were serotype Ⅰ, and 2 (6.25%) serotype Ⅱ. Of these 32 sera, 30 were determined by RT-PCR genotype method. HCV genotypes Ⅱ,Ⅲ and Ⅱ/Ⅲ were 26 (87.67%),3(10%), and Ⅰ(3.33%), respectively. The coincidence rate of the two methods was 93.33% (28/30).
ConclusionThe prevalence of HCV among blood donors in some areas of Shandong Province was relatively high. Plasma-donation was a risk factor of HCV infection in blood donors.The prevalent subtypes of HCV were serotype Ⅰ and genotype Ⅱ.