膀胱移行细胞癌患者D17S5位点基因频率分布特征分析

Distributing Analysis of the D17S5 Locus in Primary Bladder Transitional Cell Cancer

摘要: 目的探讨膀胱移行细胞癌患者D₁₇S₅位点的遗传多态性.方法应用PCR、聚丙烯酰胺凝胶电泳及银染法,对52例原发膀胱移行细胞癌患者进行了检测.结果发现10个等位基因位点,与正常对照群体的频率分布存在显着性差异(P<0.001);基因型频率分布也与正常对照群体不同,不符合HardyWeinberg平衡(P<0.001);膀胱移行细胞癌患者纯合子的频率显着高于对照组(P<0.01).结论膀胱移行细胞癌D17S5位点等位基因数目及基因型频率分布与对照人群不同,提示其频率分布的改变可能与等位基因的杂合性丢失有关.

Abstract: Objective To understand the genetic polymorphs of the D₁₇S₅ locus in TCC.Methods The D₁₇S₅ VNTR of 52 patients of TCC were observed by PCR,PAGE and silver staining methods.Results The analysis revealed 10 alleles,different from normal individuals significantly(P<0.001).The distribution of genotypes were in bad agreement with the expected number under the Hardy-weinberg equilibrium(P<0.001).The heterozygosity of D₁₇S₅ locus in TCC(61.54%)was less than that in normal organism(83.85%)(P<0.01).Conclusion The results suggested that the changes of the distribution of D₁₇S₅ VNTR might be due to deletions of some frequencies or LOH.