早期矽肺BAL液中肺表面活性蛋白A和磷脂含量研究

Surfactant Protein A and Phospholipid Levels in BAL Fluids of Silicosis Patients

摘要: 目的为探讨早期矽肺患者BAL液中表面活性物含量改变及其意义.方法对矽肺0+期组13人和Ⅰ期5人,以及健康农民对照7人,进行经纤支镜支气管肺泡灌洗收集BAL液,测定表面活性蛋白A(SPA)和磷脂(PL)及其组分含量.结果矽肺0+期组和Ⅰ期组SPA和磷脂及组分含量与对照组比较,差异无显着性意义,矽肺Ⅰ期组PG/PI低于对照组,矽肺0+期组SPA/PL高于对照组,差别有显着性意义(P<0.05).结论本研究表明,BAL从中SPA/PL升高,PG/PI降低是矽肺的早期效应指标.

Abstract: Objective To explore the biomarkers of early silicosis in the BAL fluids.By measuring surfactant protein A and phospholipid level.Method The 13 term 0+ and 5 term Ⅰsilicosis patients were studied according to x-ray diagnosis.And 7 healthy smoking peasants were selected as a control group.The BAL fluid with fibro endoscopy was collected and the levels of surfactant protein A(SP-A)and phospholipid(PL)levels in the BAL fluids were measured with ELISA and HPLC.Result The levels of SP-A,PL in BAL fluid in 0+ and Ⅰ term silicosis group had no differ ences to that in the control group.The SP-A/PL in 0+ term group was higher and the PG/PI in term group was less respectively than those values in the control group.Conclusion The increase of SP-A/PL and decrease of PG/PI in BAL may be effect biomarkers for early silicosis.