免疫-PCR检测HBsAg方法的建立及临床应用

Establishment and Application of Immuno-PCR for the Detection of HBsAg

摘要: 目的建立一种免疫聚合酶链反应技术.方法与ELISA相比较,对其敏感性、特异性、重复性进行试验研究.结果该法检测HBsAg的最低限是1pg/ml,敏感性是ELISA法的800倍,且特异性强,重复性好.应用此方法对51例乙肝患者血清中的HBsAg进行检测,阳性率为17.6%(9/51).结论此方法敏感性高、特异性强、重复性好,对乙肝的诊断、治疗及预后均有重要意义.

Abstract: ObjectiveImmuno-PCR was established.Sensitivity、specifity and repetition were detected in standard of HBV, comparing with ELISA.ResultsThis assay offer a senitivity of 1pg/ml,800 fold more sensitive than conventional ELISA,the positive percentage of ser um HBsAg in 51 cases of HB patients was 17.6% (9/51).ConclusionImmuno-PCR for serum HBsAg is a high specificty、sensitivity and repetition.It is significant in clinical diagnosis treatment and prognosis of HB.