蜂胶对环磷酰胺等4种诱变剂诱发突变的抑制作用

Antagonistic Effect of Propolis on Mutations Induced by CP, 2-AF, NaN3, DRN

摘要: 目的探讨蜂胶对环磷酰胺等强诱变剂诱发基因突变的抑制作用.方法应用Ames试验分别检测蜂胶对环磷酰胺(CP)、叠氮钠(NaN₃)、正定霉素(DNR)、2-氨基芴(2-AF)诱发突变的抑制作用.结果蜂胶对CP等4种诱变剂诱发的突变均产生抑制作用,且伴有剂-反应关系;其中8～200μg/皿浓度蜂胶均出现中等强度或以下的抑制(抑制率<75.0%),1000μg/皿浓度蜂胶对2-AF、NaN₃、CP等3种诱变剂出现了强度抑制(抑制率分别为80.9%,79.7%,98.7%).蜂胶本身无诱变活性.结论蜂胶对CP等4种诱变剂诱发的不同类型的基因突变均有一定的抑制作用.

Abstract: Objective To investigate the antagonistic effects of propolis on mutations induced by cyclophosphamide et.powerful mutagens.Methods Apply Ames test to assess the antagonistic effect of propolis to mutation induced by cyclophosphamide(CP),2 Aminofluorane(2 AF),sodium azide(NaN₃),Daunorubicin(DNR).Results The propolis inhibit all the mutations induced by the four kind of mutagens and have dose effect relationship.Dose of 8～200μg/plate of the propolis could inhibit mutations by the middle intensity or below(restrain rate75.0%).At dose of 1000μg/plate of propolis could strongly inhibit mutations induced by CP,2 AF,NaN₃(restrain rate are 80.9%,79.7%,98.7% respectively).Conclusion Propolis could antagonize the gene mutations induced by cyclophosphamide,2 Aminofluorane,sodium azide,Daunorubicin.