维生素C拮抗氟抑制体外培养淋巴细胞抗氧化酶活性的研究
Effect of Vitamin C Antagonism on Fluorine-induced Inhibition of Antioxidant Enzymes Activities in Human Lymphocyte in Vitro
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摘要: 目的研究氟对体外培养淋巴细胞抗氧化酶活性的影响以及维生素C的保护作用.方法测定氟和维生素C分别或共同对体外培养淋巴细胞中的SOD、GSH-Px、CAT的影响.结果50μmol/L的NaF即可对淋巴细胞内的三种抗氧化酶有不同程度的抑制作用(P<0.05).10μmol/LVc可不同程度的拮抗NaF对SOD和CAT的抑制作用(P<0.05),但只有Vc浓度达到40μmol/L时,才能拮抗NaF对GSH-Px的抑制作用(P<0.05).Abstract: Objective To study the effect of vitamin C antagonism to fluorine-induced inhibition of antioxidant enzymes activities in human lymphocyte in vitro.Methods SOD,GSH-Px,CAT were determined in human lymphocyte in vitro after adding fluorine or vitamin C and fluorine.Results Three kind of antioxidant enzyme were inhibited with 50μmol/L fluorine;10 μmol/L vitamin C can antagonize fluorine-induced inhibition of SOD,CAT;while 40 μmol/L Vc do the same to GSH-Px.
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