我国SEO型汉坦病毒L基因核苷酸序列测定研究

Study on L Gene Necleotide Sequence of Hantaan SEO Type Virus in China

摘要: 目的为各基因型或亚型病毒生物学表型研究打基础,对疫苗的选择使用提供科学依据.方法将获得SEO型病毒分离、MCABS分型RT-PCR扩增及核苷酸序列测定.结果首次测出我国SEO型病毒部分L基因核苷酸序列,并与76-118、C4、L99、Seoul株的核苷酸及氨基酸同源性进行比较,分别为70.8%、72%、95.3%、95.9%及75.6%、75.1%、97.7%、97.3%.结论病毒基因组间的重排不是造成发病高峰的原因.

Abstract: ObjectiveTo lay basis for studing and to provide scientific basis for selecting vaccine SEO viral genes.MethodsViral isolation MCABS typing and RT-PCR were used to determinate a SEO strain.Nucleotide sequence of its L gene was detected.ResultsThe nucleotide sequence of partial L region of SEO virus was acquired for the first time in China.Compared with 76-118 C4 L99 and Seoul strain,the homologies of nucleotide sequence were 70.8%、72%、95.3%、95.9%,respectively,amino acid sequences were 75.6%、75.1%、97.7%、97.3% repectively.ConclusionThe high epidemics of HFRS wasn't because of recombinant of viral genes.