Abstract: Objective To study the effect of selenium,cadmium supplement on the contents of Se、Cd、Cu、Zn、Mn in rat organs.Methods Wistar male rats,3 months old,were treated with the water which contained 5ppm selenium,50ppm cadmium and 5ppm selenium+50ppm cadmium.After three weeks,the contents of Se、Cd、Cu、Zn and Mn in the liver、Kidney、spleen and testicles were determined by atomic absorption spect rophotometry.Results In 5ppm Se group,Se and Cd contents in four kinds of tissues,Zn contents in liver and spleen,and Cu contents in kidney、spleen、testicles were higher than those of control group.In 50ppm Cd group:Cd contents in four kinds of tissues,Se contents in kindney and spleen,Cu and Zn contents in liver and spleen were higher than those of control group.Supplement on Se and Cd at the same time,Se contents in four kind of tissues of rat were significantly lower than those of Se group,While Cd contents in four rat tissues were significantly higher,Zn contents in liver were obviously lower than those of Cd group and Se group in spleeen;Cu contents in kidney、pleen、testicles were lower than those of Se group;Mn contents in kidney and testicles were lower than those of Cd group.