Abstract: Objective Early semi-quantitative detection of HIV-1 nef RAN level.Methods In the same reaction,the primers nef 6 and nef 7 for nef gene and the primers BA 1,BA 4 for gene β-actin were used to detect the samples with reverse polymerase chain rection.BIO-1D software was used to analyze the density of the bands and get the semi-quantitition of HIV-1 nef RNA level.Results The amplified gene production of HIV-1 nef and β-actin were showed in seven samples,and the difference among 7 samples were obtained by the analysis of BIO-1D software.Conclusion This method was sensitive,simple and economic,and was one of the optimal method in study of HIV-1 early replication and HIV pathogenesis.