肝癌p53基因等表达异常及相关性研究

Expression and Related Analysis of Protein53, AFP, CEA in Hepatocelluar Carcinomas and Side Carcinomas

摘要: 目的从分子生物学及病理学角度对肝癌中抑癌基因p53和肿瘤标志物AFP(甲胎蛋白)、CEA(癌胚抗原)的表达及相关性进行研究.方法收集40例肝细胞(HCC)患者癌组织连带部分癌旁组织,用免疫组织化学法检测p53基因、AFP、CEA等.结果 p53基因癌内表达率为80%,癌旁组织为50%;AFP癌内表达率为87.5%,癌旁组织为80%;CEA癌内表达率为55%,癌旁组织表达不明显.结论检测p53基因,并与其它肿瘤标志物和相关基因一起,在基因水平诊断肝癌、估计预后及制定术后治疗方案等有着重要作用.

Abstract: Objective Studying the expresses and their rleation of p53,AFP and CEA in carcinoma tissues and carcinoma sides tissues.Methods Carcinoma tissues and carcinoma side tissues was collected from 40 cases HCC,p53,AFP,CEA was detected with LsAB.Results p53 positive rates in carcinoma tissues was 80%,in carcinoma sides tissues was 50%;AFP rates in carcinoma tissues was 87.5%,in carcinnoma tissues was 80%;CEA rates in carcinoma sides tissues was 55%,in carcinoma sides tissues was not obviously.Conclusion As a results,the expressions of p53 with related genes in carcinoma tissues can indicate their malignant degrees.