BSA-ELISA法检测血清中瀔草及藜草花粉特异性IgE
Detection of Serum Specific IgE for Humulus and Chenopodium Pollens with BSA-ELISA
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摘要: 目的 提高瀔草及藜草花粉过敏性哮喘病因学诊断的可靠性.方法 应用BSAELISA法,检测62例哮喘患者及30例健康成人血清中瀔草与藜草花粉特异性IgE(sIgE),与临床皮肤试验(ID)结果比较.结果 瀔草及藜草花粉ID与sIgE同属,阴性和阳性反应者均85%以上,瀔草与藜草花粉sIgE及ID阳性率均45%以上;瀔草与藜草花粉sIgE测定OD指数均值明显高于正常人,P<0.01;健康成人ID阳性率67%,sIgE无1例阳性.结论 瀔草及藜草花粉是西安地区哮喘患者重要吸入性致敏原,具有较强变应原性,可使敏感者体内产生较高水平的slgE;ID及sIgE测定均为瀔草及藜草花粉过敏性哮喘病因诊断的可靠方法,尤其是BSAELISA法测定sIgE的特异性更强.Abstract: Objective To improve the accuracy of etiologic diagnosis of asthma caused by the pollens of humulus and chenopodium.Methods Serum sIgE of humulus and chenopodium was measured with BSAELISA in 62 cases with asthma and 30 healthy controls,and the results were compared with that of ID.Results The positive rate of ID and specific IgE for humulus and chenopodium were all over 45%.The coincident positive and negative rate in the results of ID were both over 85%.The mean number of specific IgEOD index was also much higher than that of controls(P<0.01);The positve rate of ID was 6.7% in controls,but none of them was positive for sIgE.Conclusion The pollens of humulus and chenopodium were important inhalant allergens causing asthma in Xi'an area.High serum level of sIgE was found in those who were allergic to humulus and chenopodium with strong allergnicity.Detections for the ID and sIgE especially with BSAELIDA were reliable methods in the diagnosis of allergic asthma caused by humulus and chenopodium pollens.
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