Abstract: Objective To improve the isolating rate of influenza viruses and influenza surveillance effect,two methods for isolating influenza viruses were compared.Methods Five hundred throat swab samples from patients suffering cold were inoculated on cell culture,and into chick-embryo allantoic and amniotic cavity in the same time.Results Thirty five influenza virus strains were obtained by the method of cell culture(7%),12 strains by the chick-embryo technics(2.4%).Of all the influenza virus strains,15 strains were type A3 and 20 strains type B.Conclusion The MDCK cell cultrue is more sensitive than the chick-embtyo technics in isolating influenza viruses.By using the two methods of cultutre in the same time,the isolating rate of influenza viruses can be obviously raised.