Abstract: Objective To provide some laboratory data for using ozone solution as a disinfectant,the influences of peptone on germicidal efficiency of ozone solution were observed using generator provided by Guoyuan Technological Institute on Disinfection of Beijing.Methods According to the procession and requirement in "Technological Specification on Disinfection(the third version): The first handbook: Technological Specification on Experiment" (1999.12 ),germicidal experiment were taken with method of fix-quantity in solution,concentration of ozone was measured with iodine-quantity method.Results The residual ozone in ozone solution and the germicidal effieiency of ozone solution could be strongly lowred by presence of 1% peptone.Increase in the volume of the ozone solution to 50ml,the killing rates could reach to 99.93% after lmin.Conclusion Considering the strong influence of organic substance on ozone solution,the volume of the ozone solution should be increased when using ozone solutlon as a disinfectant in order to assure the germicidal effieiency in actual use.