微量快速检测HFRS病毒特异性抗体的自身血凝实验

Micro Simple Rapid Autologous Erythocyte Agglutination Assay for Detection of Antibodies Against Hanta Virus

摘要: 目的验证微量快速自身血凝实验用于检测肾综合征出血热(HFRS)病毒抗体的实用性.方法采用直接玻片自身血凝试验检测临床诊断为HFRS病人全血中的特异性抗体,采用间接免疫荧光法作平行对照.结果检测HFRS病人全血54份,自身血凝实验49例(90.7%)为阳性,而间接免疫荧光仅45例(83.33%)阳性;用自身血凝实验检测90例正常人全血,其中89例不出现凝集.结论检测HFRS病人全血中病毒抗体的自身血凝实验是一种简便,微量,快速的试验诊断方法,非常适合基层医疗单位使用.

Abstract: Objective To study the possibility of autologous erythocyte agglutination assay(AGEN) to detect antibodies against Hanta Virus.Methods HFRS patients' whole blood were detected using AGEN and IFAT.AGEN test and IFAT were compared.Results Forty nine of 54 (90.8% ) HFRS whole blood samples were positive by AGEN test; Ninety normal persons' whole blood samples were tested by AGEN,89 cases were negative.Conclusion The AGEN test was a new micro,simple,rapid and economic method to detect antibodies against Hanta Virus.