氯化镉诱导293细胞凋亡的实验研究

Study on Apotosis of 293 Cells Induced by Cadmium Chloride

摘要: 目的采取体外细胞培养方法观察CdCl₂在不同时间、剂量作用下,诱导293细胞凋亡关系.方法实验中选择0,12.50,25.0,50.0μmol/L共4种浓度CdCl₂在体外与293细胞共育不同时间后(12h,24h,48h),采用台盼蓝拒染法测定细胞存活率,观察293细胞转化,用DNA凝胶电泳及ELISA方法检测细胞凋亡.结果 12.5,25.00,50.0μmol/L CdCl₂可诱导细胞凋亡;不同浓度CdCl₂可引起细胞存活率下降,与CdCl₂时间、剂量有关.结论 CdCl₂在体外可诱导293细胞凋亡,与CdCl₂的作用时间、作用浓度相关.

Abstract: Objective To explain apoptosis induced by cadmium chloride in 293 cells in vitro.Methods 293 cells were exposed to 0,12.5,25.0,50.0μmol/L of CdCl₂ for various time period,to measure the apoptosis by DNA agarose electrophoresis and ELISA.Results CdCl₂ could induce apotosis of 293 cells in vitro at concentration of 12.50,25.00,50.00μmol/L.Different concentration of CdCl₂ could also cause reduction of cells survial associated with exposed doses and time and apoptosis.Conclusion Cadmium chloride coucld induce 293 cell apoptosis in vitro associated with exposed dose and time.