中国HIV-1感染者相关基因SDF1、CCR2b、CCR5多态性分析

Analysis on Polymorphism of SDF1, CCR2b, CCR5 Related to HIV-1 Infection of Chinese HIV-1 Infected Population

摘要: 目的分析91例中国HIV-1感染人群SDF1、CCR2b、CCR5等位基因型的多态性和分布特点。方法应用聚合酶链反应(PCR)、限制性片段长度多态性(RFLP)分析技术和核酸测序技术分析HIV-1感染人群SDF1、CCR2b、CCR5基因多态性。结果未发现91例中国HIV-1感染者有CCR5△32突变型基因,SDF1-3'A、CCR2b-64I等位基因突变频率分别为26.40%、2.43%,核酸测序结果与PCR/RFLP分析结果一致。结论91例中国HIV-1感染者均为易感者,中国HIV-1感染者SDF1、CCR2b、CCR5不同基因型分布特点,提示,中国人群可能对HIV-1有较大易感性。

Abstract: ObjectiveTo analyse the frequency and polymorphism of HIV-1 resistant SDF1,CCR 2b,CCR5 alleles in 91 HIV-1 infected Chinese persons.MethodsPCR/RFLP assay technology and DNA sequencing were used to analyse gene poly mor phism of SDF 1,CCR2b,CCR5 among HIV-1 infected population.ResultsccR5△32 gentype was not found in 91 HIV-1 infected Chinese persons.The frequencies of SDF1-3cA and CCR2b-64I alleles were 26.40% and 21.43%.DNA sequencing had the same results with PCR/RFLP.ConclusionThese 91 HIV-1 infected Chinese persons were all HIV genetic susceptive person.The frequencies and polymorphism of HIV-1 resistant SDF 1,CCR2b,CCR5 alleles in Chinese HIV-1 infected population were analyzed,which can help further study how SDF1,CCR2b and CCR5 gene polymorphism affected the course of AIDS of Chinese population.