Abstract: ObjectiveTo analyze the epidemic characteristics,sources of infection,risk factors and trend of HIV infection in Jiangsu province.MethodsHIV antibody was detected by ELISA and WB in the serum of high risk groups.The anticongulated blood of the infected people was collected to separate previrus DNA.Then it was amplified between C2-V3 region by nPCR.The amplified products were sequenced to identify HIV-1 subtypes.ResultsFrom J anuary 1986 to the end of J une 2001,a total number of 954 445 serum samples were detected for HIV antibody.One hundred and thirty-three people with HIV-1 sero-positive were identified,accounting for 0.14‰ of the total numbers.There were 8 patients with AIDS in which 4 cases died.Four subtypes of group M HIV-1 were found in Jiangsu:A,B',C,D.Subtype B'was circulating among commercial blood donors,subtype C was among IDUs,while subtype A and D were among labourers returned from abroad.ConclusionThe number of HIV/AIDS cases increased rapidly in recent years in Jiangsu.The major transmission route were through IDUs and sexual contacts.HIV-1 subtypes were complex.It was necessary to conduct comprehensive STD/AIDS surveillance and behaviour intervention of high risk groups.