乙肝病毒标志物与PCR-HBV定量水平的关系

Preliminary Study on Relationships Between HBV Immunological Marker and HBV-DNA Concentrations

摘要: 目的研究乙型肝炎病毒标志物定量检测结果与其HBV-DNA含量的临床关系.方法采用时间分辨免疫定量和荧光定量-PCR技术,对HBV-M和HBV-DNA含量进行检测.结果在HBsAg/HBeAg/HBcAb,HBsAg/HBeAb/HBcAb,HBeAg/IC及单纯HBcAb阳性的4个组中,HBV-DNA阳性率分别是97.17%,30.56%,100%和25%;在HBsAg,HBeAg,HBeAb、HBcAb定量检测高、低值两组HBV-DNA含量对比中,HBV-DNA阳性率分别为76.92%,58.14%;100%,97.26%;20.00%,11.42%;64.62%和27.63%.结论 HBV-DNA比HBV-M更能及时准确反映乙型肝炎病毒感染者的病程情况.

Abstract: Objective To investigate the relationships between HBV infected patients' immunological marker and HBV-DNA concentrations.Methods TRFIA and FQ-PCR methods were applied in the analysis of HBV immunological marker and HBV-DNA concentrations in 216 HBV infected patients.Results In patients with HBsAg+/HBeAg+/HBcAb+,HBsAg+/HBeAb+/HBcAb+,HBeAg+/IC+,and HBcAb+,the HBV-DNA positive rate were 97.17%,30.56%,100%,25%.In the high and low titer groups about HBsAg+,HBeAg+,HBeAb+,and HBcAb+,the HBV-DNA positive rate were 76.92%,58.14%;100%,97.26%;20.00%,11.42%;64.62%,27.63%.Conclusion HBV-DNA was more rapid and exact than HBV immunological marker to reflect the HBV infected patients.