HPLC法同时测定肉与肉制品中沙丁胺醇和8种雌激素残留量

Determination on Residue of Salbutamol and 8 Kinds Estrogen in Meat and Meat Products by HPLC

摘要: 目的建立HPLC法测定肉与肉制品中沙丁胺醇、β-雌己醇、炔雌醇、己烯雌酚、双烯雌酚、己雌酚、戊酸雌二醇、苯甲酸雌二醇、炔雌醚残留量的方法.方法样品经甲醇超声萃取、离心,提取液浓缩后经0.45μmFH滤膜过滤,进高效液相色谱分析.结果 9种组分得到很好分离,并得到浓度与峰面积成正比的工作曲线,相关系数r>0.999;其变异系数CV≤1.1%(n=6),检出限≤6ng,回收率范围为84.5%～101%.结论该法具有灵敏、准确、精密、样品处理简单、无杂质干扰等优点.

Abstract: Objective To study a method for the determination of Salbutamolβ,-Estradiol,Ethinylesradiol,diethylstilbestrol,Dienestrol Hexestrol,Estradiol valerate,Estradiol benzoate and Quinestrol residue in meat and meat products by HPLC.Methods Components were extracted from homogenized sample with methanol,then the HPLC method was used to analyze them.Results Good separation was achieved in the 9 kinds components and the standard curve showing a direct ratio between the concentration and the peak area was obtained,r>01999,CV≤1.1%(n=6);the detection limits was below 6ng;The recovery percent was between 84.5% and 101%.Conclusion The method was proved to be satisfactory in precision,securacy and sensitivity.The preparation of sample was simple.Nothing intrefered determination of the 9 kinds component.