锌营养对大鼠应激反应及金属硫蛋白的影响

王冬兰; 程义勇; 洪燕; 李树田; 耿战辉

doi:10.11847/zgggws2003-19-04-01

锌营养对大鼠应激反应及金属硫蛋白的影响

Effects of zinc nutriture on stress-response and metallothionein in rats

摘要

摘要:
目的在心理应激条件下,观察大鼠组织中金属硫蛋白(MT)水平的变化及其与锌营养状况的关系.

方法 .60只雄性Wistar大鼠随机分为6组:缺锌组(ZD)、喂对组(PF)、对照组(CT),以及相应的3个应激组(SZ、SP、SC).以光-电刺激方法建立心理应激动物模型,观察应激对皮质醇激素、锌和金属硫蛋白的影响.

结果应激诱导动物脑和肝脏MT含量增加,而血浆锌出现不同程度下降.缺锌动物血锌浓度降低,肝脏和脑中金属硫蛋白的含量减少,而其整体应激反应强度增加.

结论维持良好的锌营养状况有助于诱导整体和脑组织产生应激蛋白,并提高机体对心理应激的适应能力.

Abstract:
Objective To obser ve the changes of metallothionein and cortisol of rats in different zincnutriture under psychological condition.

Methods Sixty rats were divided into six groups:zinc deficient(ZD),pair-fed(PF),control(CT)and the corresponding 3 stress groups(SZ,SP,SC).The psychological stress was induced by light-electric stimuli.

Results The stressor induced more MT synthesis in brain and liver tissues while the zinc concentrations decreased to some extent in the stress animals.The zinc concentrations in sera,the MT contents in both liver and brain were decreased while the cortisol levels were increased in ZD groups.

Conclusion It was indicated that maintaining adequate zinc nutriture was beneficial to induce the synthesis of MT in brain and liver of the rats,and to enhance their adapting ability to psychological stress.

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