锰抑制多巴胺能神经细胞SN4741生长研究

徐文; 陈景元; 王枫; 骆文静; 缪珊

doi:10.11847/zgggws2003-19-04-15

锰抑制多巴胺能神经细胞SN4741生长研究

Toxicity effects of manganese on growth and cell cycle of SN4741 cells

摘要

摘要:
目的研究不同剂量锰的毒性对多巴胺能神经细胞SN4741生长抑制作用的特点, 探讨锰损伤神经细胞的作用机理.
方法取对数生长期神经细胞株SN4741, 使用含有200, 400, 600, 800μmol/LMnCl₂的培养液, 分别作用1, 2, 4, 6d后, 用噻唑蓝(MTT)比色试验检测细胞生存活力; 台盼蓝染色法绘制细胞生长曲线; 筛选锰的细胞毒性剂量; 细胞周期和凋亡细胞DNA特异性荧光染色观测细胞周期及凋亡; 透射电镜观察细胞形态学变化.
结果 200, 400, 600, 800μmol/LMnCl₂作用2, 4, 6d均对SN4741细胞株有显着的抑制作用, 且呈剂量和时间效应关系.流式细胞仪检测结果表明: 600μmol/LMnCl₂作用6天可将SN4741细胞生长周期阻滞在S期, 并且诱导凋亡, 这与电镜结果一致.
结论诱导凋亡可能是锰对神经细胞增殖抑制作用的重要机制.

Abstract:
Objective To study the effects of different doses manganese on neurocyte, SN4741.
Methods SN4741 was incubated in culture media with 200, 400, 600, 800μmol/L manganese(MnCl₂)for 2 days, 4 days and 6 days respectively.The cell growth livingness was examed by MTT and cell growth curve was made.The cell cycle was monitor ed by flow cytometry(FCM), at the same time, morphological changes was investigated by transmisssion electron microscope.
Results Manganese of different concentrations(MnCl₂ 200, 400, 600, 800 μmol/L)could suppress the proliferation of SN4741 cells in dose and time-dependent manner.FCM showed that the cell cycle of the SN4741 cells could be inhibited in Speriod at certain observed through flow cytometry(FCM)and transmission electron microscope.
Conclusion The apoptosis of SN4741 cell induced by mang anese(MnCl₂)may be its important mechanism on proliferation inhibition.

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