氟化物对大鼠成骨细胞增殖分化的影响

Effects of fluoride on proliferation and differentiation of osteoblast in rats

摘要:
目的研究不同浓度氟化物对体外培养大鼠成骨细胞增殖和分化的影响.
方法大鼠颅骨分离的成骨细胞经不同浓度(10μmol/L~4mmol/L)的氟化钠作用后, 采用AlamarBlueTM摄入法检测细胞增殖; ELISA方法测定碱性磷酸酶活性.
结果低浓度氟化钠(100μmol/L~1mmol/L)在体外可刺激细胞增殖, 高浓度氟化钠(2mmol/L以上)可抑制细胞增殖; 10μmol/L~50μmol/L的NaF增强细胞ALP活力, 100μmol/L以上的NaF降低了细胞ALP活力.
结论氟化物对成骨细胞的增殖与分化具有双向作用, 即低浓度促进, 高浓度抑制.

Abstract:
Objective Effect of fluoride on proliferation and differentiation of osteoblast in rats was studied in vitro.
Methods Various concentration of sodium fluoride(NaF)were added to the osteoblasts.The proliferative response to NaF was determined by the percents of reduced Alamar Blue.The Activity of alkaline phosphatase(ALP)was measured by Elisa method.
Results NaF increased the proliferation of rat osteoblast at low doses(100 μmol/L-1 mmol/L), whereas athigh doses(≥2 mmol/L)it decreased the proliferation; ALP activity was increased at 10-50 μmol/L, and decreased at ≥100 μmol/L.
Conclusion Fluoride had two-phase effects.At low doses it promoted osteoblast.s proliferation and differentiation while athigh doses ithad a negative effect.