肉类食品细菌污染生物发光快速分析技术研究

Rapid biolu minescent technique to detect bacteria in meat

摘要:
目的建立快速准确的肉类食品细菌污染的检测方法.
方法利用ATP生物发光分析技术和体细胞清除技术, 测量细菌ATP和体细胞ATP, 细菌ATP量与细菌数成正比.
结果对于鲜肉类食品, 须先清除体细胞ATP, 分析测量时间需15 min.对于熟制品, 无需清除体细胞, 其分析测量时间只需4 min.测量细菌的下限为10³cfu/g.
结论利用ATP生物发光分析技术检测肉类食品细菌污染, 可实现快速适时的检测, 是一项值得广泛推广应用的新技术.

Abstract:
Objective To establish a method to dete mine the bacteria pollution of meat rapidly.
Methods The microbial ATP and the somatic ATP are measured by using ATP-biolu minescence technique and removing somatic ATP technique.The microbial ATP content is directly proportional to bacteria count.
Results The somatic ATP of raw meat must be removed, so the measurement of the micobial ATP content of raw meat takes bout 15 min.The somatic ATP of the cooked meat is released and hydrolyzed, so the detecting time of microbial content of these cooked cured foods can be reduced to 4 min.In the procedure applied detection limit is about 10³ cfu/g.
Conclusion The bacteria polluting situation of meat can be deter mined rapidly and timely by using A TP-biolu minescence technique.The technique can be widely used to foods sanitation.