HBsAg阳性孕妇及新生儿HBV复制状况的研究

Study on replication status of HBsAg positive pregnant women and newborns

摘要:
目的探讨HBsAg阳性孕妇及其新生儿外周血单个核细胞乙肝病毒(PBMCHBV)复制状况。
方法收集2001年6月~2002年12月由太原市传染病院妇产科进行产前检查并分娩的151例HBsAg阳性孕妇及其153例新生儿, 用选择性聚合酶链反应技术进行PBMCHBVrcDNA与PBMCHBVcccDNA的检测。
结果 HBsAg阳性孕妇PBMCHBVrcDNA的检出率为36.42%(55/151), PBMCHBVcccDNA的检出率为9.93%(15/151);新生儿PBMCHBVrcDNA的检出率为22.88%(35/153), PBMCHBVcccDNA的检出率为4.58%(7/153)。
结论 PBMC为HBV的肝外复制场所, 新生儿PBMCHBV复制与其母亲体内PBMCHBV复制有关。

Abstract:
Objective In order to explore the replication status of PBMC HBV of HBsAg positive pregnant women and their newborns.
Methods 151 pregnant women and 153 newborns were collected between 2001 June and 2002 December in Infectious Hospital of Taiyuan city. PBMC HBV DNA were deter mined by selected poly merase chain reaction (sPCR).
Results 15(9.93%) out of 151 HBsAg positive pregnant women had HBV cccDNA in their PBMC, 7(4.58%) out of 153 newborns had HBV cccDNA in their PBMC, 55(36.42%) out of 151 HBsAg positive pregnant women had HBV rcDNA in their PBMC, 35(22.88%) out of 153 new borns had HBV rcDNA in their PBMC.
Conclusion PBMC was the replication site of HBV out of liver. PBMC HBV replicatlon of newborns was related to the replication of their mothers.