纳米SiO2对大鼠肺脏白介素-4表达的影响
Effect of nanosized silicon dioxide on expressions of interleukin-4 and fibroblast growth factor-b in lungs of rat
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摘要:目的 研究纳米SiO2对大鼠肺脏白介素-4(IL-4)和成纤维生长因子-b(FGF-b)表达的影响。方法 气管内非暴露法染尘, 一次性分别给予雌性Wistar大鼠纳米SiO2〔粒径(10±5)nm〕和常规SiO2粉尘(粒径 < 5μm)20mg/只, 分别于染尘1个月和2个月时处死动物。HE和VG染色观察实验动物肺组织病理改变, 免疫组化卵白素-生物素过氧化物酶复合物(ABC)法观察动物肺组织IL-4和FGF-b的表达情况。结果 (1) 纳米SiO2组大鼠的肺纤维化程度(1期)明显轻于常规SiO2组(Ⅰ+~Ⅲ期)。(2)纳米SiO2组大鼠肺脏IL-4表达程度稍高于对照组, 与常规SiO2组比表达程度较轻。1个月时, 纳米SiO2组和常规SiO2组IL-4表达程度均重于2个月。而FGF-b无论在纳米SiO2组或常规SiO2对照组, 1个月或2个月时, 其表达均为弱阳性, 与对照组无明显区别。结论 纳米SiO2致肺纤维化程度较轻, 大鼠肺脏IL-4的表达水平较低。FGF-b在大鼠的肺纤维化过程中可能无明显作用。Abstract:Objective To study the effect of nanosized silicon dioxide on expression of IL-4 (interleukin-4) and FGF-b (fibroblast growth factor-b) in silicotic lungs of rats.Methods Female Wistar rats were exposed to lower dosage (20mg/rat) of nanosized silicon dioxide and normal silicon dioxide by intratracneal injection. Rats were killed on the 1st month and 2nd month respectively to observe the pathological changes of silicotic rats by means of HE and VG dyeing and expression of IL-4 and FGF-b by means of immunohistochemical technique.Results (1) Pathological examination showed that pulmonary fibrosis lesions of rat lung were much lighter in nanosized silicon dioxide groups than that of normal silicon dioxide groups. (2) The expression of IL-4 and TGF-β1 in nanosized silicon dioxide groups was higher than control groups and lighter than normal silicon dioxide groups. The expression of IL-4 was heavier on the 1st month than on the 2nd month both in nanosized silicon dioxide groups and normal silicon dioxide groups. And the expression of FGF-b was similar to both the first and the second month and in both normal silicon dioxide groups and nanosized silicon dioxide groups.Conclusion The effect of pulmonary fibrosis lesions was light in nanosized silicon dioxide. The expressions of IL-4 in rats lungs were low. FGF-b may have no effect on the fibrosis of the lungs of rats.
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