神经酰胺诱导人结肠癌细胞凋亡的作用

Cell apoptosis induced by ceramide in human colon cancer cells

摘要:
目的探讨外源性神经酰胺诱导结肠癌HT-29细胞凋亡的作用。
方法采用噻唑兰(MTT)显色法、荧光显微镜、电镜观察以及通过免疫组化方法检测Fas、P53的表达情况。
结果神经酰胺对HT-29细胞的生长有明显抑制作用, 并可诱导HT-29细胞产生凋亡, 并随着神经酰胺浓度的增加, 细胞凋亡率逐渐增加; Fas蛋白的表达随着神经酰胺浓度的增加呈现逐渐升高的趋势, p53蛋白的表达随着神经酰胺浓度的增加呈现降低的趋势。
结论神经酰胺能诱导HT-29细胞产生凋亡。

Abstract:
Objective The effect of cell apoptosis induced by ceramide was investigated in human conlon cells(HT29).
Methods The curve of cell growth(MTT test)was used. Cell apoptosis was detected under fluorescent and electronic microscope and expressed bcl-2, Fas, p53 protein.
Results Ceramide inhibited remarkably the cell growth and induced cell apoptosis. The expression of Fas protein in HT-29 cells increased as the dose increased and the expression of p53 protein decreased as the dose increased.
Conclusion Ceramide can significantly inhibit the growth of HT-29 cells and induce apoptosis.