支链氨基酸对大鼠TPHmRNA表达的影响
Effects of branched-chain amino acids spplementation on expression of TPH mRNA in rats
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摘要:目的 探讨补充支链氨基酸(branched-chaina minoacids, BCAA)对睡眠剥夺(sleepdeprivation, SD)大鼠脑干色氨酸羟化酶(Tryptophan hydroxylase, TPH)mRNA表达的影响.方法 采用小站台水环境(flower-pot)睡眠剥夺模型对大鼠进行睡眠剥夺.将56只雄性成年Sprague-Dawley大鼠按体重随机分为C(对照组, 自由睡眠)、24hSD(剥夺睡眠24h)、24hSDB(睡眠剥夺24h, 进食添加3% BCAA的饲料)、48hSD、48hSDB、72hSD和72hSDB组, 每组8只.睡眠剥夺结束后, 断头取脑, 冰上分离出脑干, 半定量RT-PCR法检测TPHmBNA的表达.结果 24和48h睡眠剥夺未明显改变大鼠脑干TPHmRNA的表达, 与对照组比较差异无统计学意义(P > 0.05);而72h睡眠剥夺后TPHmRNA表达量大幅度增加, 与其它各组相比差异有统计学意义(P < 0.01).补充BCAA与未补充BCAA组比较, 大鼠脑干TPHmRNA的表达水平末见明显变化.结论 一定时间的睡眠剥夺可以引起大鼠脑干TPHmRNA的表达上调, 但补充一定剂量的支链氨基酸并不能改变睡眠剥夺大鼠脑干TPHmRNA的表达.Abstract:Objective To explore the effects of branched chain amino acids(BCAA)supplementation on the expression of TPH mRNA in the brain stem of rats subjected to sleep deprivaton(SD).Methods Sleep deprivation was induced in male Sprague Dawlay rats by employing flower pottechnique.Fifty six rats were divided into seven groups randomly: C(control group), 24h SD(being sleep depr ived for 24 hours), 24 h SDB(being sleep depr ived for 24 hours and fed fodderenriched with BCAA), 48h SDB, 48h SDB, 72 h SD, 72 h SDB.Results Expressions of TPH mRNA in the brain stem in 24 h and 48 h sleep deprived groups were not significantly different from that in the control group(P > 0.05), but increased sig nificantly after 72 h sleep deprivation(P < 0.01).Expressions of TPH mRNA in the groups supplemented with BCAA were no significantly changed compared with the groups without BCAA supplementaton.Conclusion 72 hour sleep deprivation enhanced the expressons of TPH mRNA in rat brain stem; oral supplementation of BACC could not alter the change of the expressions of TPH mRNA in the brain stem of rats sbjected to sleep deprivation.
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