甲醛、三氯乙烯、三氯化铝的细胞联合毒作用

Combination toxicology of formaldehyde, trichloroethylene and alchlor with Chinese Hamster Lung Cells

摘要:
目的研究甲醛、三氯乙烯和三氯化铝的联合毒作用类型.
方法噻唑蓝法(MTT)测定3种物质各自的半数生长抑制浓度(IC₅₀)及混合物(甲醛、三氯化铝、三氯乙烯)的IC₅₀, 根据Finney数学模型和Logistic回归方法判断联合作用类型.
结果甲醛、三氯乙烯、三氯化铝及其混合物质的IC₅₀分别为0.0226, 0.368, 0.589和0.124mg/ml, 据Finney法求得Q值为1.08, Logisitc回归模型参数β4差异无统计学意义.
结论甲醛、三氯化铝、三氯乙烯(6:11:33)混合染毒, 对中国仓鼠肺细胞的联合毒性表现为相加作用.

Abstract:
Objective To study the combined action of formaldehyde, trichloroethylene and alchlor.
Methods 50% inhibition concentration(IC₅₀)and 95% confidence limits of formaldehyde, trichloroethylene, alchlorand mixture(formaldehyde, trichloriethylene and alchlor)were assessed using Methyl thiazolyl tetrazolium(MTT)with Chinese Hamster Lung Cells.Combined action of above mixture was evaluated accordingto Finney model and Log istic Regression Method.
Results IC₅₀ of formaldehyde, trichloroethylene, alchlorand the mixture were 0.0226 mg/ml, 0.368 mg/ml, 0.589 mg/ml and 0.124 mg/ml respectively.The Qvalue was 11.08 and Parameter B4 from Logistic Regression Method showed no significant.
Conclusion Combination tox icolog y of above mixture(formaldehyde: trichloroethylene: alchlor=6:11:33)belonged to additive effect.