急性染铅小鼠海马脑片凋亡相关酶活性变化

Changes of activities of apoptosis-related enzymes in hippocampal slice in acute lead-poisoned mice

摘要:
目的探讨急性铅中毒时小鼠海马钙蛋白酶和钙凋磷酸酶活性的变化及D-2-氨基-5-磷酸基戊酸(D-(-)-2-Amino-5-phosphono pentanoi cacid, AP-5)的影响.
方法采用离体海马脑片观察急性铅中毒时钙蛋白酶和钙凋磷酸酶活性的变化及AP-5的作用.
结果急性铅中毒可致海马钙蛋白酶和钙凋磷酸酶活性增加, AP-5可拮抗铅的这种作用.
结论 AP-5可拮抗铅引起的海马钙蛋白酶和钙凋磷酸酶活化.

Abstract:
Objective To explore changes of hippocampal calpain and calcineurin activities in Pb²⁺ damaged mouse and effect of(D-(-)-2-A mino-5-phosphonopentanoic acid)AP-5 on it.
Methods By means of hippocampal slice to observe changes of calpain and calcneurin act ivities in mouse.
Results Pb²⁺ resulted in increases of calpain and calcineur in activities in mouse hippo campal slices, and AP-5 could antagonize preceding changes.
Conclusion AP-5 can antagonize the increase of calpain and calcineurin activities of mouse induced by Pb ²⁺.