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徐健, 林汉华, 孙秀发. 甲状腺功能与大鼠肝脏葡萄糖转运子2的变化[J]. 中国公共卫生, 2005, 21(4): 442-443. DOI: 10.11847/zgggws2005-21-04-35
引用本文: 徐健, 林汉华, 孙秀发. 甲状腺功能与大鼠肝脏葡萄糖转运子2的变化[J]. 中国公共卫生, 2005, 21(4): 442-443. DOI: 10.11847/zgggws2005-21-04-35
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XU Jian, LIN Hanhua, SUN Xiufa. Changes of glucose transporter 2 in rat livers with different thyroid state[J]. Chinese Journal of Public Health, 2005, 21(4): 442-443. DOI: 10.11847/zgggws2005-21-04-35
Citation: XU Jian, LIN Hanhua, SUN Xiufa. Changes of glucose transporter 2 in rat livers with different thyroid state[J]. Chinese Journal of Public Health, 2005, 21(4): 442-443. DOI: 10.11847/zgggws2005-21-04-35
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甲状腺功能与大鼠肝脏葡萄糖转运子2的变化

Changes of glucose transporter 2 in rat livers with different thyroid state

    摘要
  • 摘要:
      目的   检测葡萄糖转运子2(GLUT2)在不同甲状腺功能状态大鼠肝脏中的变化, 以探讨其在甲亢所致的糖耐量异常中的作用.
      方法   将40只雄性Wistar大鼠随机分为4组.动物模型的诱导为甲减组; 每只他巴唑10mg/d灌胃, 轻、重度甲亢组: 每只左旋甲状腺素灌胃剂量分别为100, 200μg/d, 对照组: 每只生理盐水2ml/d灌胃.共28d.采用放射免疫法检测大鼠空腹血浆胰岛素及T3、T4浓度, 葡萄糖氧化酶法检测大鼠空腹血浆血糖, 免疫印迹技术测定大鼠肝脏细胞膜上GLUT2的浓度.
      结果   轻、重度甲亢组血糖(BG)、胰岛素(INS)、肝细胞质膜的GLUT2浓度均较对照组显著升高, 胰岛素敏感指数(IAI)显著下降, 重度甲亢组INS、GLUT2较轻度甲亢组显著升高, IAI显著下降, BG亦有升高趋势; 甲减组与对照组比较BG、INS有上升趋势, 但差异无统计学意义; IAI无显著变化, 甲减组GLUT2较对照组显著性下降.BG、INS、-IAI与GLUT2均有显著性线性正相关关系.
      结论   肝脏细胞膜上GLUT2浓度的改变可能是引起甲亢糖代谢紊乱的机制之一.

     

    Abstract:
      Objective   To study the effect of g lucose transpor ter 2(GLUT2)on impaired g lucose tolerance in hyperthy ro id rat.
      Methods   For ty male Wistar rats were divided intofour groups: hy pothyroidism, lighthyperthyroidism, serious hy per thyroidism and normal groups.Every group included ten rats.Induction of hypo or hyperthyroidism: Every hypothy roidism rat received 10 mg methimazole every day.Every lighthyperthyroidism rat received 100 μg L-thyrox ine while every serious hyperthy roidism rat received 20μg L-thyrox ine everyday.The control group received saline everv day.After 28 days, the concentrations of blood glucose, insulin, T3 and T4 were examined and GLUT2 were detected by Western blot.
      Results   The concentrations of blood glucose, insulin and GLUT2 increased significantly in light and serious hy perthyroidism rats while IAI decreased significantly.Compared with lishthyperthyroidism, GLUT2 and blood tnsulin increaseed while IATdecreased sig nificantly in serious hyperthyro idism.The concentrations of blood glucose, insulin and IAI in hypothyroidism did not change significantly.The concentrations of GLUT2 in the hypothyroidism rats decreased significantly.Blood glucose, insulin andIAI had positive sig nificant correlation with GLUT2.
      Conclusion   The changes of GLUT2 concentrations in the liver cell membrane probably contr ibuted to abnormal g lucose metabolism in hyperthyroidism.

     

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