洛伐他汀对转染乳腺癌细胞增殖功能的影响

王芳; 糜漫天; 韦娜; 罗远琼; 杨剑

doi:10.11847/zgggws2005-21-04-85

洛伐他汀对转染乳腺癌细胞增殖功能的影响

Effects of lovastatin on proliferation function of BRCA1-transfected breast cancer cells

摘要

摘要:
目的探讨胆固醇合成抑制洛伐他汀(Lovastatin, LOV)对人乳腺癌易感基因1(Breastcancer1, BRCA1)转染的密西根癌症基金会-7乳腺癌细胞(Michigan Cancer Foundation-7breast cancer cell, MCF-7)增殖功能的影响.
方法应用基因转染技术获得BRCA1高表达的MCF-7乳腺癌细胞(MCF-7^BRCA1), 经8μmol/LLOV处理未转染组(MCF-7)与转染组(MCF-7^BRCA1)细胞1~3d后, 通过生长曲线、二甲基偶氮唑蓝(MTT)染色和透射电镜等方法, 观察LOV对MCF-7及MCF-7^BRCA1乳腺癌细胞增殖功能的影响.
结果 LOV处理乳腺癌细胞均能抑制未转染组和转染组细胞的增殖, 以MCF-7^BRCA1细胞的变化更为显著.透射电镜结果显示, LOV能明显诱导MCF-7^BRCA1转染组细胞向正常细胞的分化.
结论乳腺癌细胞BRCA1基因的高表达可增强LOV对细胞的增殖抑制作用.

Abstract:
Objective To study the effect of cholesterol inhibitor lovastatin on the proliferation function of BRCA1 transfected MCF 7 breast cancer cells.
Methods BRCA 1 gene overexpressing MCF 7 human breast cancer cell(MCF-7^BRCA1)were generated by gene transfectingtechnique.After beingtreated with 8 mol/L lovastatin for 1-3 d, the prolifera tion of nontransfected(MCF-7)cells and BRCA 1 transfected(MCF-7^BRCA1)cell were analyzed by growth curve, MTTand transmission electron microscope.
Results Lovastatin could inhibit the proliferation of MCF-7 cells and MCF-7^BRCA1 cells, but the changes was more obviously in MCF-7^BRCA1 cells than MCF-7 cells.Mean while, lovastatin could significantly induce the differentiation of MCF-7^BRCA1 cells.
Conclusion The overexpression of BRCA1 gene in MCF-7 cells coulden hance the funcion of inhibiting cells proliferation by lovastatin.

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