三羟异黄酮拮抗表皮生长因子促乳腺癌细胞增殖
Effects of EGF and genistein on proliferation of human breast cancer cell
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摘要:目的 通过体外细胞, 观察三羟异黄酮(genistein)和表皮生长因子(EGF)对乳腺癌MCF-7细胞增殖的影响。方法 用不同剂量genistein、不同剂量EGF、固定剂量genistein和EGF的混合液, 作用于体外培养的雌激素受体阳性(ER +)人乳腺癌细胞株MCF-7, 作用不同时间后, 用噻唑蓝(MTT)法测定细胞生长情况。结果 genistein剂量超过25μmol/L以后对人乳腺癌细胞株MCF-7细胞增殖均有抑制作用, 而且随剂量增大和作用时间延长其抑制作用逐渐增强, 其中50μmol/L为最适宜浓度, EGF剂量超过4.15nmol/L以后对人乳腺癌细胞株MCF-7细胞增有明显的促进作用, 而且随剂量增大和作用时间延长其作用逐渐增强, 其中8.30nmol/LEGF联合作用于MCF-7细胞, 则可显示出两者有交互作用, EGF对细胞的促增殖作用可以被genistein的作用逆转。结论 genistein可抑制EGF诱导的人乳腺癌细胞株MCF-7细胞的增殖。Abstract:Objective To observe the effect of genistein and epidermal growth factor(EGF)on the proliferation of human breast cancer cell MCF-7 in vitro.Methods Cell growth status was observed with methyl thiazolyl tetrazolium (MTT)when it was cultured with different doses of genistein or EGF for different time respectively, or with definite dose of genistein and EGF.Results The growth of MCF-7 was inhibited when it was exposed to genistein over 25 μmol/L seemed to be an optional dose.Cell growth was greatly promoted when it was exposed to EGF over 4.15 nmol/L, the more the dose and time became, the greater the promoting effect was, and 8.30 nmol/L seemed to be an optional dose.When MCF-7 was cultured with 50 μmol/L genistein and 8.30 nmol/L EGF simultaneously, an interaction effect was observed.The promoting effect of EGF was reversed by genistein.Conclusion Genistein can inhibit the proliferation of human breast Cancer cell MCF-7 induced by EGF.
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