细胞色素P4502E1基因多态性与胃癌易患性
Effects of genetic polymorphism of cytochrome P4502 E1 on susceptibility to gastric cancer
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摘要:目的 研究与前致癌物亚硝胺类代谢活化有关的细胞色素P4502E1(cytochrome P4502E1, CYP2E1)基因多态性与胃癌易患性的关系, 探讨环境因素与遗传因素在胃癌发病中的作用。方法 采用病例-对照分子流行病学方法, 调查原发性胃癌患者和对照各48例, 以聚合酶链反应-限制性片段长度多态(PCR-RFLP)检测被调查者的CYP2E1基因RsaI位点的多态性, 条件Logistic回归模型进行资料分析。结果 CYP2E1A、B和C3种基因型在病例组中的构成比分别为68.8%, 29.2%和2.1%, 对照组分别为45.83%, 47.92%和6.25%, 2组差异无统计学意义(χ2=5.389, P=0.068)。而病例和对照组CYP2E1基因RsaI位点等位基因c1和c2频率分别为83.3%, 16.7%和69.8%, 30.2%, 差异有统计学意义(χ2=5.241, P < 0.05)。环境因素中食盐、香肠和熟食的过多摄入以及肿瘤家庭史和c1等位基因作为胃癌的危险因素进入回归模型, 而经常喝茶作为保护性因子可能降低胃癌的发生危险。结论 CYP2E1基因RsaI位点等位基因c1与胃癌易感性相关联; 某些饮食因素与胃癌的发生有关。Abstract:Objective To investigate the relationship between genetic polymorphisms of cytochrome P450 2E1 (CYP2E1)and the susceptibility to gastric carcinoma, and to study the action between environmental and genetic factors in the development of gastric carcinoma.Methods The Rsa Ⅰ poly mophism of CYP2E1 of 48 patients with gastric cancer and 48 controls were detected by PCR-RFLP.Related information of environmental factors were collected and analyzed by using the multiple-variable Logistic regression models.Results The frequency distribution of 3 genotype of CYP2E1 were 68.8%, 29.2% and 2.1% in cases and 45.83%, 47.92% and 6.25% in controls(χ2=5.389, P=0.068).The frequencies of the allelec1 and c2CYP2E1 were 83.3%, 16.7% respectively in cases and 69.8%, 30.2% respectively in controls.Gastric cases were more likely to carry allelec1 than controls.(χ2=5.241, P < 0.05).Multi variable stepwise logistic regression showed thathigh intake of salt, sausage and fried food were the risk factors for gastric cancer, while consumption of tea was the protective factors for gastric cancer.Conclusion Genetic polymorphism of cytochrome P4502 E1 and some environmental factors were significantly related with the risk of gastric cancer.
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