花青素对人脐静脉内皮细胞基质金属蛋白酶影响

Effects of anthocyanin on matrix metalloproteinase expression in human umbilical vein endothelial cell

摘要:
目的研究花青素对人脐静脉内皮细胞基质金属蛋白酶(MMP)1, 2和9蛋白表达和活性的影响。
方法分离人脐静脉内皮细胞, 先用5mg/L人重组CD₄0配体(rCD40L)处理细胞24h, 再分别用1, 10, 100μmol/L的花青素标准品矢车菊定3葡萄糖苷(C3G)和芍药定3葡萄糖苷(P3G)处理细胞24h。用Western Blotting分别检测MMP-1, MMP-2, MMP-9的蛋白表达量, 用酶谱分析法检测MMP-1, MMP-2, MMP-9的活性水平。
结果花青素C3G和P3G组的MMP-1, MMP-2, MMP-9的活力明显下降, 且蛋白表达量也明显下降。
结论花青素C3G和P3G能够降低rCD40L诱导的人脐静脉内皮细胞MMP-1, MMP-2, MMP-9的蛋白表达和活性水平, 对于增加动脉粥样斑块的稳定性有重要意义。

Abstract:
Objective To explore the effect of ant hocyanin on matrix metalloproteinases(MMP)expression and activity.
Methods Human umbilical vein endot helial cell were isolated and incubated with 5μg/mL rCD40L for 24 h.After that, the endot helial cell were incubated with 1μM, 10μM, 100μM C3G and P3G for another 24 h, respectively.
Methods of zymography and Western blotting were used to analyze MMP-1, MMP-2, MMP-9 activities and protein expression.
Results Ant hocyanin C3G and P3G inhibited MMP-1, MMP-2 and MMP-9 protein expression and their activities significantly.
Conclusion Ant hocyanin C3G and P3G inhibited MMP-1, MMP-2, MMP-9 protein expression and their activities.