砷诱导Jurkat T淋巴细胞铁蛋白重链的表达

吴顺华; 郑玉建; 张跃新; 成军; 刘开泰; 王国荃

doi:10.11847/zgggws2005-21-06-21

砷诱导Jurkat T淋巴细胞铁蛋白重链的表达

Cloning of ferritin H chain gene from human Jurkat T lymphocyte induced by arsenic trioxide

摘要

摘要:
目的了解三氧化二砷诱导人JuikatT淋巴细胞的差异表达基因, 探讨砷对淋巴细胞基因转录的影响机制。
方法在体外用三氧化二砷(5μmol/L, 24h)处理人Jurkat T淋巴细胞后, 应用抑制性消减杂交技术(SSH)和基因克隆技术构建差异表达的cDNA文库, 用PCR技术和测序技术鉴定阳性克隆。
结果用SSH技术构建了由三氧化二砷诱导的人JurkatT淋巴细胞的差异表达基因的正向消减cDNA文库。经测序分析结果显示, 铁蛋白重链在正向消减cDNA文库中有2个阳性克隆。
结论三氧化二砷可诱导铁蛋白重链的表达, 铁蛋白重链在淋巴细胞抗砷损伤机制中可能起着一定的作用。

Abstract:
Objective To understand the differentially expressed genes and to explore the effects on mechanism of gene transcription in human T lymphocytes induced by arsenic trioxide.
Methods Human J urkat T cell line as cell model was treated by arsenic trioxide(5μmol/L, 24 h)in vitro.Then the differential expressed genes were cloned and the subtractive cDNA library from Jurkat T cell line was constructed by suppression subtractive hybridization.The PCR and sequencing techniques had been applied to testive clones.
Results The forward subtracted cDNA library from J urkat T cell line induced by arsenic trioxide was constructed.Sequencing analysis showed that there were two clones contained ferritin H chain in the forward subtractive cDNA library.
Conclusion Arsenic trioxide can induce the transcription of ferritin H chain in T lymphocytes, and the ferritin H chain may play certain role in the mechanism of anti-arsenical cytotoxicity.

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