甲基叔丁基醚对小鼠外周血T淋巴细胞亚群影响

邓学良; 贺性鹏; 唐国华; 龙鼎新; 陈时植

doi:10.11847/zgggws2005-21-08-21

甲基叔丁基醚对小鼠外周血T淋巴细胞亚群影响

Effect of methyl tertiary butyl ether on peripheral T lymphocyte subsets of mice

摘要

摘要:
目的探讨汽油添加剂甲基叔丁基醚对小鼠外周血T淋巴细胞亚群的作用。
方法 30只健康昆明种成年小鼠, 体重(28.5±2.9)g, 随机分为3组, 灌胃染毒, 染毒剂量分别为400, 100和0mg/(kg·bw)。每天1次, 共计30d。应用流式细胞仪测定外周血T淋巴细胞亚群CD₃、CD₄和CD₈计数的变化, 并计算胸腺及脾脏指数。
结果甲基叔丁基醚染毒组体重轻度降低。可使外周免疫器官胸腺重及胸腺指数降低及外周血淋巴细胞亚群CD₃、CD₄减少, 经统计学检验差异有统计学意义(P < 0.05)。
结论甲基叔丁醚可引起外周血淋巴细胞亚群CD₃、CD₄减少, 免疫功能抑制。

Abstract:
Objective To explore the effect of methyl tertiary butyl ether(MTBE), an unleaded gasoline additive, on peripheral Tlymphocyte subsets of mice.
Methods 30 mice were distributed in three groups randomly.The MTBE exposed doses were 40, 100 and 0 mg/(kg·bw)respectively by gavage daily for 30 days.The change of the body weight, pectoral gland weight and spleen weight was detected.The percentage of peripheral T lymphocyte subsets CD₃, CD₄ and CD₈ cells was analyzed by flow cytometer.
Results The body weights in MTBE exposed groups were decreased lightly than those of control group.The weight of pectoral gland and the index of pectoral gland, T lymphocyte subsets CD₃, CD₄, cells in MTBE 400mg/kg group were significantly decreased compared with control group P < 0.05).
Conclusion MTBE presented adverse effects on the T lymphocyte subsets and suppressed immunefunction.

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