高级检索
刘晓莉, 杨东升, 孟紫强. 大气细颗粒物对大鼠脑组织的氧化损伤效应[J]. 中国公共卫生, 2005, 21(8): 990-991. DOI: 10.11847/zgggws2005-21-08-51
引用本文: 刘晓莉, 杨东升, 孟紫强. 大气细颗粒物对大鼠脑组织的氧化损伤效应[J]. 中国公共卫生, 2005, 21(8): 990-991. DOI: 10.11847/zgggws2005-21-08-51
shu
LIU Xiaoli, YANG Dongsheng, MENG Ziqiang. Oxidation damage of airborne fine particulate matter on brains of rats[J]. Chinese Journal of Public Health, 2005, 21(8): 990-991. DOI: 10.11847/zgggws2005-21-08-51
Citation: LIU Xiaoli, YANG Dongsheng, MENG Ziqiang. Oxidation damage of airborne fine particulate matter on brains of rats[J]. Chinese Journal of Public Health, 2005, 21(8): 990-991. DOI: 10.11847/zgggws2005-21-08-51
shu

大气细颗粒物对大鼠脑组织的氧化损伤效应

Oxidation damage of airborne fine particulate matter on brains of rats

    摘要
  • 摘要:
      目的   探讨大气细颗粒物(PM2.5)对大鼠脑组织的毒性作用机制。
      方法   将32只雄性Wistar大鼠随机分为低、中、高(1.5, 7.5, 37.5mg/kg)3个剂量染毒组和生理盐水对照组。气管注入染毒后24h处死大鼠, 测定脑组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性和谷胱甘肽(GSH)、硫代巴比妥酸反应物(TBARS)含量。
      结果   经PM2.5染毒后大鼠脑组织的SOD、CAT活性出现显著的剂量依赖性下降趋势; 高浓度组GSH含量显著降低; GSH-Px活性在不同剂量染毒后虽出现下降趋势, 脂质过氧化水平(LPO)也有所升高, 但与对照组之间差异无统计学意义。各染毒组SOD/TBARS比值显著降低(P < 0.05), TBARS/GSH-Px比值则在高浓度染毒后较对照组明显升高(P < 0.05)。
      结论   PM2.5可引起大鼠脑组织的氧化损伤, 是一种神经毒性因子。

     

    Abstract:
      Objective   To investigate the toxicological mechanism of airborne fine particulate(PM2.5)on brains of rats.
      Methods   32 male wistar rats were randomly divided into PM2.5 exposure groups at different concentration(1.5, 7.5, 37.5 mg/kg)and control group instilled with physiological saline.Rats were killed 24 h after instillation, and the levels of thiobar bituric acid reactive substance(TBARS), Glutathione(GSH)and activities of superoxide dismutase(SOD), Glutathione peroxidase(GSH-Px), Catalase(CAT)were measured.
      Results   The activities of SOD and CAT decreased with PM2.5 concentration in a dose depenent manner(P < 0.05).PM2.5 instillation at 37.5 mg/kg caused statistically significant decrease of GSH level.(P < 0.05).The changes of GSH-Px activities and TBARS levels at all concentration were not statistically significent in comparison with control group.SOD/TBARS decreased significantly at all concentrations, and TBARS/GSH-Px increased significently at 37.5 mg/kg concentration(P < 0.05).
      Conclusion   PM2.5 could cause oxidative damage to brains of rats.It was a neural toxin.

     

    • HTML全文
    • 参考文献(9)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回