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李燕, 梁彩云, 高凯, 顾菁, 徐慧芳. 265例HIV-1抗体阳性者抗体蛋白印迹带型分析[J]. 中国公共卫生, 2005, 21(11): 1293-1294. DOI: 10.11847/zgggws2005-21-11-08
引用本文: 李燕, 梁彩云, 高凯, 顾菁, 徐慧芳. 265例HIV-1抗体阳性者抗体蛋白印迹带型分析[J]. 中国公共卫生, 2005, 21(11): 1293-1294. DOI: 10.11847/zgggws2005-21-11-08
LI Yan, LIANG Caiyun, GAO Kai, . Assay of western blot bands pattern of antibody of 265 samples HIV-1 antibody positive[J]. Chinese Journal of Public Health, 2005, 21(11): 1293-1294. DOI: 10.11847/zgggws2005-21-11-08
Citation: LI Yan, LIANG Caiyun, GAO Kai, . Assay of western blot bands pattern of antibody of 265 samples HIV-1 antibody positive[J]. Chinese Journal of Public Health, 2005, 21(11): 1293-1294. DOI: 10.11847/zgggws2005-21-11-08

265例HIV-1抗体阳性者抗体蛋白印迹带型分析

Assay of western blot bands pattern of antibody of 265 samples HIV-1 antibody positive

  • 摘要:
      目的   通过对本实验室以蛋白印迹(WB)法确认的HIV-1抗体阳性者抗体WB条带的分析, 了解不同人群抗体WB带型分布的差异.
      方法   WB法确认HIV-1抗体阳性.
      结果   抗pol基因编码蛋白的抗体WB带型分布以及P31抗体的阳性率在不同传播途径的HIV-1抗体阳性者间差异有统计学意义(P < 0.01);年龄(age, A)≤40岁的HIV-1抗体阳性者P66和P24抗体阳性率分别明显高于相应A > 40岁以上者, (P < 0.05);抗pol基因编码蛋白的抗体WB带型分布以及P66抗体阳性率在不同地域的HIV-1抗体阳性者间差异有统计学意义(P < 0.01).
      结论   HIV-1抗体WB带型分布在不同性别、不同年龄人群间差异无统计学意义, 但在不同传播方式、不同地域的人群间差异有统计学意义; HIV-1 pol、gag基因编码的某些蛋白的抗体产生在不同传播方式、不同年龄及不同地域的人群间差异有统计学意义.

     

    Abstract:
      Objective   To explore the difference of western blot(WB)bands pattern in various HIV-1 polsitive people by investig ate the WB bands of HIV-1 antibody.
      Methods   People diag nosed as HIV-1 posotive person by WB.
      Results   The WB bands pattern of antibody resistantigen coded by pol gene and the positive rate of P31 antibody has significant difference between the people infected by different transmission mechanism, P < 0.01;The positive rate of P66 and P24 antibody of people less than orequal to 40 years was significant greater than thatof people older than 40 years, P < 0.05;The WB bands pattern of antibody resistantigen coded by polgene and the positive rate of P 66 antibody has significant differnce among people from various regions.
      Conclusion   HIV-1 antibody WB bands pattern is not significant diffferent among people who are different sexor age, butitis different among people who are infected through differenttrans mission mechanism or from various region; the production of some antibody resistantigen coded by HIV-1 polor gaggene is different among people who are infected through different transmission mechanism or from various region or differentage.

     

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