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才秀莲, 李兴升, 李季蓉, 张先平. 染锰鼠睾丸增殖细胞核抗原与热休克蛋白表达[J]. 中国公共卫生, 2005, 21(11): 1331-1332. DOI: 10.11847/zgggws2005-21-11-30
引用本文: 才秀莲, 李兴升, 李季蓉, 张先平. 染锰鼠睾丸增殖细胞核抗原与热休克蛋白表达[J]. 中国公共卫生, 2005, 21(11): 1331-1332. DOI: 10.11847/zgggws2005-21-11-30
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CAI Xiulian, LI Xingsheng, LI Jirong, . Expression of proliferation cell nucleus antigen and heat-shock protein of testis in mice exposed to manganese[J]. Chinese Journal of Public Health, 2005, 21(11): 1331-1332. DOI: 10.11847/zgggws2005-21-11-30
Citation: CAI Xiulian, LI Xingsheng, LI Jirong, . Expression of proliferation cell nucleus antigen and heat-shock protein of testis in mice exposed to manganese[J]. Chinese Journal of Public Health, 2005, 21(11): 1331-1332. DOI: 10.11847/zgggws2005-21-11-30
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染锰鼠睾丸增殖细胞核抗原与热休克蛋白表达

Expression of proliferation cell nucleus antigen and heat-shock protein of testis in mice exposed to manganese

    摘要
  • 摘要:
      目的   研究锰对小鼠精子数量与睾丸增殖细胞核抗原(PCNA)和热休克蛋白70(HSP70)表达的影响.
      方法   将小鼠随机分为3个不同剂量MnCl2(7.5, 15, 30 mg/kg)组和对照组, 分别于染锰, 3, 7, 14, 28, 56 d计数精子数量, 用免疫组化S-P法结合图象分析技术测定睾丸PCNA和HSP70表达.
      结果   随染锰剂量增加和时间延长精子数量减少、睾丸PCNA表达降低.3个不同剂量MnCl2组精子计数于染锰28 d明显低于对照组(P < 0.05, P < 0.01, P < 0.01), 睾丸PCNA表达于染锰14, 28, 56 d比对照组明显降低(P < 0.05, P < 0.01, P < 0.01), 直线相关分析各染锰组小鼠精子数量与睾丸PCNA表达呈正相关(r=0.794, P < 0.01).睾丸HSP70表达, 15, 30 mg/kg组染锰56 d明显低于染锰28 d(P < 0.01).
      结论   锰对小鼠生精功能的损伤呈一定的剂量-效应和时间-效应关系.各染锰组小鼠精子数量与睾丸PCNA表达呈正相关(r=0.794, P < 0.01).锰可诱导小鼠睾丸HSP70表达, 染锰28 d达高峰.

     

    Abstract:
      Objective   To studyeffects of manganese on countof sperm and expression of prolifer ation cell nucleus antig en(PCNA)and hea-tshock protein 70(HSP70)of testis in mice.
      Methods   The mice were randomly divided into three mang anese chloride(MnCl2)(7.5, 15, 60 mg/kg)groups and control group.Exposed to manganese for 3, 7, 14, 28, 56 days, quantity of sperm was counted.The expression of PCNA and HSP70 in testis of mice was analysed by immunohistochemical technique(S-P)and the image analysis.
      Results   The countof sperm and expression of PCNA and HSP70 of testis decreased with the increasing of dose and time exposed to manganese.The countof sperm in three groups MnCl2 were much lower than those of control group(P < 0.05, P < 0.01, P < 0.01)28 days afterexposure, and the expression of PCNA were much lower than those of control groups(P < 0.05, P < 0.01, P < 0.01)14, 28, 56 days afterexposure.The countof sperm and expressions of PCNA of all exposed groups were correlated positively with each other(r=0.794, P < 0.01).The expression of HSP 70 in testis of 15 and 30 mg/kg groups 56 days afterexposure were much lower than those of 28 days afterexposure(P < 0.01).
      Conclusion   There are dos-eeffects and time-effectin the damage of sper matog enic functions in mice exposed to manganese.The countof sperm and expression of PCNA of all exposed groups are correlated positively with each o ther(r=0.794, P < 0.01).The increase of HSP70 expression can be induced by Mncl2 and peaked 28 days afterexposure.

     

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